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Plasmid#207483PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertRFP, NY-ESO-1_TCR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
Plasmid#109428PurposeA lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.DepositorInsertmCherry T2A GFP
UseLentiviralTagsExpressionMutationInserted 43 base pairs into mCherry to create rep…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
W118-1_Flag-hIRAK1
Plasmid#180405Purposelentiviral transduction of human IRAK1 (with an N-terminal Flag tag) geneDepositorInsertIRAK1 (IRAK1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_Cre
Plasmid#201198PurposeAAV vector for cre expression under EF1 promoterDepositorInsertscre
EF1alpha promoter
UseAAVTagsExpressionBacterial and MammalianMutationnuclear targeting crePromoterN/A and hEF1alpha promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 B.1.351v3
Plasmid#169464PurposeEncodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 B.1.1.7
Plasmid#169459PurposeEncodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES-BSD
Plasmid#209751PurposeLentiviral transfer plasmid to express the blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP SNAP47
Plasmid#101915PurposeExpresses SNAP47 in mammalian cellsDepositorInsertSNAP47 (SNAP47 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTwist-SARS-CoV-2
Plasmid#164435PurposeEncodes SARS-CoV-2 Spike Protein for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pHDM-SARS-CoV-2
Plasmid#164433PurposeEncodes SARS-CoV-2 Spike Protein for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-HEK3_del1-5-NGG
Plasmid#185478PurposepegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.DepositorInsertHEK3_del1-5-NGG pegRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP
Plasmid#176027Purposeconstruct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometryDepositorInsertsrtTA3
Luc2
mtagBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-GFP-LoxP-SNAP-TERT
Plasmid#71391PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
IGF2BP1_2xRRM-4xKH_pGEX
Plasmid#135122PurposeRecombinant expression of IGF2BP1 with dual-affinity tagDepositorInsertIGF2BP1 (IGF2BP1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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H14-MBP-SUMO-Cys-Linker-BAF_G47E
Plasmid#101779PurposeExpresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationG47E mutationPromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only