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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2799

Plasmid

#63658

Purpose

pCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2992

Plasmid

#63642

Purpose

pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

Plasmid

#129706

Purpose

Express cre-dependent KASH-EGFP and Gmeb1 shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GTL-y

Plasmid

#81100

Purpose

Gene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBER

Plasmid

#62662

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

tdTomato
hygromycin

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GTL-ir

Plasmid

#81102

Purpose

Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

tdiRFP

Use

Cre/Lox

Tags

tdiRFP

Expression

Yeast

Mutation

codon optimized second iRFP to avoid internal rec…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GTT-y

Plasmid

#81110

Purpose

Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMB84 (Nf2 C-term)

Plasmid

#12169

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Nf2 C-terminal half (Nf2 Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

C-terminal half of Nf2 cDNA

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO-PBG-WPRE-hGH

Plasmid

#74287

Purpose

pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

Insert

PBG (chimeric rabies Glycoprotein)

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO-mCherry_Dicer gRNA

Plasmid

#215279

Purpose

Expresses Dicer gRNA in a Cre dependent mcherry vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Dicer control gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO-mCherry_DicerTTT gRNA

Plasmid

#215280

Purpose

Expresses Dicer Control gRNA in a Cre dependent mcherry vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Dicer control gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-C+F-ArchT-GFP

Plasmid

#209700

Purpose

Expresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

ArchT-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output; derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Article

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

CAG

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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GTU-c

Plasmid

#81093

Purpose

Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

yemCFP

Use

Cre/Lox

Tags

yemCFP

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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