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Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR wt-FLAG
Plasmid#204525PurposeRetroviral expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR del52-FLAG
Plasmid#204523PurposeRetroviral expression of human CALR del52-FLAGDepositorInsertCALR del52-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationdel52PromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti/TO/EYFP-P2A-IDH1(HA)
Plasmid#122482PurposeA lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HADepositorInsertisocitrate dehydrogenase 1 (IDH1 Human)
UseLentiviralTagsHA and P2AExpressionMammalianMutationPromoterCMV/TOAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X* (AcrIIC1X-mCherry chimera)
Plasmid#128114PurposeExpresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9DepositorInsertAcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR ins5-FLAG
Plasmid#204524PurposeRetroviral expression of human CALR ins5-FLAGDepositorInsertCALR ins5-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationins5PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del18-197)-FLAG
Plasmid#204539PurposeMammalian expression of human CALR ins5 (del18-197)-FLAGDepositorInsertCALR ins5 (del18-197)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del18-197)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del198-430)-FLAG
Plasmid#204538PurposeMammalian expression of human CALR ins5 (del198-430)-FLAGDepositorInsertCALR ins5 (del198-430)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del198-430)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del367-430)-FLAG
Plasmid#204536PurposeMammalian expression of human CALR ins5 (del367-430)-FLAGDepositorInsertCALR ins5 (del367-430)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del367-430)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del309-430)-FLAG
Plasmid#204537PurposeMammalian expression of human CALR ins5 (del309-430)-FLAGDepositorInsertCALR ins5 (del309-430)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del309-430)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del1-308)-FLAG
Plasmid#204535PurposeMammalian expression of human CALR ins5 (del1-308)-FLAGDepositorInsertCALR ins5 (del1-308)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del1-308)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del198-308)-FLAG
Plasmid#204540PurposeMammalian expression of human CALR ins5 (del198-308)-FLAGDepositorInsertCALR ins5 (del198-308)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del198-308)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del1-197)-FLAG
Plasmid#204534PurposeMammalian expression of human CALR ins5 (del1-197)-FLAGDepositorInsertCALR ins5 (del1-197)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del1-197)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR ins5 (del1-17)-FLAG
Plasmid#204533PurposeMammalian expression of human CALR ins5 (del1-17)-FLAGDepositorInsertCALR ins5 (del1-17)-FLAG (CALR Human)
UseTagsExpressionMammalianMutationins5 (del1-17)PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Acly(S455D)
Plasmid#70768PurposeExpresses phosphomimetic mouse ATP-citrate lyase in mammalian cellsDepositorInsertATP Citrate Lyase (Acly Mouse)
UseTagsmyc-epitopeExpressionMammalianMutationS455DPromoterEF1alphaAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MCU-Flag-TetOFF
Plasmid#185657PurposeTet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporterDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only