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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

p23

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Taekjip Ha, Jie Chen

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Use

Tags

HA and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CAPN1

Plasmid

#60941

Purpose

Expresses calpain1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain1 (CAPN1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAF0687 Pa01_attP-mCherry donor

Plasmid

#193466

Purpose

Promoterless mcherry donor for Pa01

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PG13-luc (wt p53 binding sites)

Plasmid

#16442

Purpose

Luciferase reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pHAGE NFkB-TA-LUC-UBC-GFP-W

Plasmid

#49343

Purpose

pHAGE NFkB-TA-LUC-UBC-GFP-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

eGFP (eGFP )
Luciferase
NFkB consensus binding sequence
TA
UBC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO GFP-MDC1 (779-2)

Plasmid

#26285

Depositor

Eric Campeau

Insert

Mediator of DNA damage checkpoint 1 (MDC1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP1-10

Plasmid

#68715

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

GFP1-10

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHJ421

Plasmid

#20680

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-nlsCRE

Plasmid

#12265

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

Cre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimised wtCNa

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti-sh1368 knockdown c-myc

Plasmid

#29435

Depositor

Bob Eisenman

Article

Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Insert

c-Myc shRNA (MYC Human)

Use

Lentiviral and RNAi

Tags

GFP and WPRE

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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FUtdTW

Plasmid

#22478

Depositor

Connie Cepko

Article

Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

tandem dimer tomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Flag p21 WT

Plasmid

#16240

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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