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Plasmid#98677PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2ADepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationN1775A, N1782APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_del1961-1970
Plasmid#98680PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970DepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationdel1961-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_N2A_S1966C
Plasmid#98682PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationN1775A, N1782A, S1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S_S1966C
Plasmid#98683PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887S, S1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S103W (#623)
Plasmid#99941Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS103WPromoterAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT1
Plasmid#79499PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDSDepositorInsertWNT1 (WNT1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK381
Plasmid#71573PurposeProduces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges aspartate-317 to glycinePromoterrecAAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK382
Plasmid#71574PurposeProduces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges aspartate-317 to asparagine; silent mutat…PromoterrecAAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK347
Plasmid#71833PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK348
Plasmid#71832PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK193
Plasmid#71834PurposeCloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) regionDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UsePhage lambda cloning vector with genomic dna inse…TagsExpressionMutationadditional N-terminal methionine encoded by this …PromoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4F04
Plasmid#71030PurposeGateway entry cloneDepositorInsertSin3A (Sin3A Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF1B10
Plasmid#70840PurposeGateway entry cloneDepositorInsertSce (Sce Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2C08
Plasmid#70911PurposeGateway entry cloneDepositorInsertTaf5 (Taf5 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2C05
Plasmid#70891PurposeGateway entry cloneDepositorInsertOrc2 (Orc2 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARA-no stop
Plasmid#67347PurposehSara without stop codon in Gateway Entry vectorDepositorInsertSARA (SAR1B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160S,HisTagDel)
Plasmid#66880PurposeExpresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160S)
UseTagsnoneExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160E,HisTagDel)
Plasmid#66879PurposeExpresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160E)
UseTagsExpressionBacterialMutationS160EPromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK R4-R3
Plasmid#62109PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK L5-L2
Plasmid#62108PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only