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pENTR1A FKBP12F36V-OGT

Plasmid

#154287

Purpose

Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x HA and FKBP12(F36V)

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-NES-L2

Plasmid

#127349

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Expression

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b CnA CnB

Plasmid

#11787

Depositor

Anjana Rao

Article

Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

Insert

CnA CnB (PPP3R1, PPP3CA Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
pDD2148

Plasmid

#153968

Purpose

pCMV6-Entry vector encoding human IRAK1 protein 196F

Depositor

Dirk Dittmer

Article

Seltzer et al J Virol. 2020 May 4;94(10). pii: JVI.021

Insert

IRAK1 (IRAK1 Human)

Use

Tags

Myc-DDK

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-NES-L2

Plasmid

#127351

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CYFIP2.mCherry

Plasmid

#122052

Purpose

Expresses human CYFIP2 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

Plasmid

#139445

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RVQ

Plasmid

#200187

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RVQ

Use

CRISPR; Gateway compatible lbcas12a-rv entry clone

Tags

NLS

Expression

Plant

Mutation

G146R, R182V, E795Q

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pATZ-Myc

Plasmid

#177718

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

Myc

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK2-S

Plasmid

#172426

Purpose

Expression of full-length, human GIRK2 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK2 (KCNJ6 Human)

Use

Tags

Streptag II

Expression

Insect

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3C

Plasmid

#137758

Purpose

Expression of LC3C

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3C (MAP1LC3C Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR223-BRLF1(Rta)

Plasmid

#195824

Purpose

Gateway Entry Clone of Epstein-Barr virus BRLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRLF1 (BRLF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT WT

Plasmid

#154282

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Expression

Mutation

WT OGT

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTrcHisB-TRF1

Plasmid

#53209

Purpose

Expressed N-terminally Hexahistidine tagged TRF1 protein

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF1 (TERF1 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

3-439

Promoter

Trc

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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PpyCAG-KLF2-IB

Plasmid

#60441

Purpose

Transient human KLF2 expression

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

KLF2 (KLF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only