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Plasmid#211611PurposesgRNA-A against RAD9DepositorInsertsgRNA RAD9A
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gRAD1_A
Plasmid#211544PurposesgRNA-A against RAD1DepositorInsertsgRNA RAD1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gHUS1_B
Plasmid#211542PurposesgRNA-B against HUS1DepositorInsertsgRNA HUS1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gIL25_A
Plasmid#211543PurposesgRNA-A against IL25DepositorInsertsgRNA IL25
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJTT002
Plasmid#204493PurposeReporter circuitDepositorInsertsTagBFP
EYFP
mKate2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-EGFP-Cre
Plasmid#207132PurposeLentiviral vector to express EGFP-CreDepositorInsertEGFP-Cre
UseLentiviralTagsExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
UseTagsExpressionMammalianMutationT27stopPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LAAPPW
Plasmid#205693PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterAHAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LBAPPW
Plasmid#205666PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterCBHAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LCAPPW
Plasmid#205648PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterhCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LHAPPW
Plasmid#205684PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterEHAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LEAPPW
Plasmid#205675PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP hSmad4-HomDonor
Plasmid#206044PurposeDonor plasmid for CRISPR knock-inDepositorInsertSmad4 (SMAD4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-COQ8B-V5-miniTurbo_IDG-K
Plasmid#189915PurposeGateway destination clone of COQ8B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCOQ8B (ADCK4 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationF435SPromoterUbiquitinAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16-18 E7 Chimera B
Plasmid#191543PurposeProtein expression of HPV16 E7 Chimera BDepositorInsertHPV16-18 E7
UseRetroviralTagsFlagHAExpressionMutationCR1 CR2 HPV18 E7, C-terminus HPV16 E7PromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16-18 E7 Chimera A
Plasmid#191542PurposeProtein expression of HPV16 E7 Chimera ADepositorInsertHPV16-18 E7
UseRetroviralTagsFlagHAExpressionMutationCR1 CR2 HPV16 E7, C-terminus HPV18 E7PromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16-18 E7 Chimera C
Plasmid#191544PurposeProtein expression of HPV16 E7 Chimera CDepositorInsertHPV16-18 E7
UseRetroviralTagsFlagHAExpressionMutationCR1 HPV18 E7, CR2 C-terminus HPV16 E7PromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MAPK4_IDG-K
Plasmid#189906PurposeGateway destination clone of MAPK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertMAPK4 (MAPK4 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only