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MO70-hCRACR2A-isoform-c

Plasmid

#79591

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

Insert

CRACR2A-isoform-c (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

Plasmid

#174336

Purpose

The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Retroviral

Tags

pHluorin inserted in the extracellular loop 2 of …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

Plasmid

#46594

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-17 Decoy (MIR17, Mir17 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pAcGFP1-N1-RvCAHS8-GS

Plasmid

#192461

Purpose

Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-132-Decoy.hPGK.GFP.WPRE

Plasmid

#46587

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-132 Decoy (MIR132, Mir132 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLJM60-S6K1(T389S) barcoded

Plasmid

#48800

Purpose

expresses mutant S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 T389S (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGTACC

Expression

Mammalian

Mutation

T389S

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Use

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159673

Purpose

Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-223-Decoy.hPGK.GFP.WPRE

Plasmid

#46601

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-223 Decoy (MIR223, Mir223 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-FT_INF

Plasmid

#112874

Purpose

binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting FT

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

Plasmid

#46590

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-146a Decoy (MIR146A, Mir146 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pBCMV-MS2CP-eDHFR

Plasmid

#167309

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

MS2CP-eDHFR

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2CP-VPg(FCV)

Plasmid

#167314

Purpose

Template pDNA to prepare CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.U6.miR-210-Decoy.hPGK.GFP.WPRE

Plasmid

#46599

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-210 Decoy (MIR210, Mir210 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-27b-Decoy.hPGK.GFP.WPRE

Plasmid

#46603

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-27b Decoy (MIR27B, Mir27b Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only