Addgene

Showing: 2781 - 2800 of 28572 results

Results per page:

Sort results by:

p2T-CAG-MCS-P2A-GFP-PuroR

Plasmid

#107186

Purpose

Base plasmid for cloning gene duplication alleles with eGFP reporter.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

Plasmid

#185451

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-ABEmax-P2A-EGFP-ires-puro

Plasmid

#121170

Purpose

CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

Depositor

Timo Otonkoski

Insert

ABEmax-P2A-EGFP-ires-puro

Use

CRISPR

Tags

P2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BioID2-1x-NSP3 pBabe-puro (SARS-CoV-2)

Plasmid

#185997

Purpose

Expresses myc-tagged BioID2 fused to SARS-CoV-2 NSP3

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 NSP3 (ORF1ab SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and myc

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT-CMV-UBE3B(C1036A)-copGFP-Puro

Plasmid

#107344

Purpose

Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A)copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

mutation C1036A

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro

Plasmid

#22135

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids from wt: Proline 405 t…

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLIP-puro-dsRed-GFP-miRNA

Plasmid

#32704

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

emGFP-miRNA

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PGK Puro GFP-LC3B G120

Plasmid

#123241

Purpose

Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

PGK

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-puro GFP-p62 K7A, D69A

Plasmid

#38281

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed K7A, D69A

Promoter

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

Plasmid

#121184

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

CMV-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-BRD9-PGK-Puro-IRES-GFP

Plasmid

#75114

Purpose

Retroviral expression plasmid encoding wild type human BRD9

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

Plasmid

#172618

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr283Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

Plasmid

#120561

Purpose

Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

Plasmid

#75122

Purpose

Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pLenti PGK Puro GFP-LC3B G120A

Plasmid

#123242

Purpose

Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

PGK

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO-mcherry-luc-puro-TRPM1 cDNA

Plasmid

#29782

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

TRPM1 (TRPM1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

Plasmid

#135814

Purpose

Fluorescent reporter for knock-in of TdTomato into the TH locus

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)

Use

Tags

p2a-TdTomato

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge