Addgene

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pGC291

Plasmid

#19684

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep . 180(1):103-19.

Insert

Phlh-12

Use

Gateway entry clone

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC450

Plasmid

#19691

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep . 180(1):103-19.

Insert

Phlh-12

Use

Gateway entry clone

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNRF1

Plasmid

#198580

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNRF1 (BNRF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Tags

Expression

Mutation

Promoter

-3mnx1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFRF3

Plasmid

#195778

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcLF1

Plasmid

#198581

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcLF1

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1

Plasmid

#166823

Purpose

Gateway pDONR vector containing NLRP1 (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-GFP

Plasmid

#198589

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

GFP

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BKRF3

Plasmid

#195790

Purpose

Gateway Entry Clone of Epstein-Barr virus BKRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BKRF3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF2

Plasmid

#195800

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF2

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR; Gateway donor

Tags

HA Tag

Expression

Mutation

D10A;H840A

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR223-BORF2

Plasmid

#195834

Purpose

Gateway Entry Clone of Epstein-Barr virus BORF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BORF2

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-6xgata2aECEbasp

Plasmid

#132968

Purpose

gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

gata2a endothelial enhancer (x6) with a b-actin basal promoter

Use

Gateway vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2

Plasmid

#67678

Purpose

Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR MCS R4-R3

Plasmid

#62086

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-mKate2

Plasmid

#67671

Purpose

Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-P2A-mKate2

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B no-stop

Plasmid

#123205

Purpose

Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A no-stop

Plasmid

#123204

Purpose

Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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