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Plasmid#173132PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj5 (kcnj5 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj6
Plasmid#173133PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj6 (kcnj6 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND
Plasmid#169963PurposepDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-EGFP-L2
Plasmid#141045PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2
Plasmid#141051PurposeMutisite Gateway mediated vector constructionDepositorInserteSpCas9(1.1)-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-FokI-dCas9-L2
Plasmid#141042PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9n-EGFP-L2
Plasmid#141044PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9n-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-L2
Plasmid#141041PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9-EGFP-L2
Plasmid#141043PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF4-2A-EGFP-L2
Plasmid#141049PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF4-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF2-2A-EGFP-L2
Plasmid#141050PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF2-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-FokI-dCas9-EGFP-L2
Plasmid#141047PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-L2
Plasmid#141040PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-NR2F2-MUT
Plasmid#138371PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutatedDepositorInsert161k human enhancer with conserved NR2F2 binding sites mutated
UseGateway compatibleTagsExpressionMutationNR2F2 sites mutatedPromoternoneAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
IH401: pMVP (L5-L4) CMV promoter
Plasmid#121726PurposepMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO102: pMVP (R4-R3) eGFP open
Plasmid#121731PurposepMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HJ601: pMVP (R4-R3) mCherry open
Plasmid#121733PurposepMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO601: pMVP (R4-R3) eCFP open
Plasmid#121735PurposepMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO401: pMVP (R4-R3) eYFP open
Plasmid#121737PurposepMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KH305: pMVP (R4-R3) βgal open
Plasmid#121739PurposepMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertβgal (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only