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Plasmid#157636PurposesMHT-113 F1 onlyDepositorInsertsMHT(1-113)-SYNZIP17
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMF044
Plasmid#157637PurposesMHT-113 F2 onlyDepositorInsertSYNZIP18-sMHT(114-230)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNK132d
Plasmid#156370PurposeExpresses heterlogous ribosomal proteinsDepositorInsertS20, S16, S1, S15 ribosomal genes
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-313 (attR4-attR3)
Plasmid#161898PurposeMultisite Gateway (attR4-attR3) vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-894 (attR4-attR2)
Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-639 (attR4-attR2)
Plasmid#161880PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-624 (attR4-attR2)
Plasmid#161879PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9
Plasmid#154925PurposeaTc inducible eCas9DepositorInserteCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060APromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9-D1135E
Plasmid#154926PurposeaTc inducible eCas9 with D1135E mutationDepositorInserteCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060A, D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-OptiCas9-D1135E
Plasmid#154928PurposeaTc inducible OptiCas9 with D1135E mutationDepositorInsertOptiCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationR661A, K1003H, D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9
Plasmid#154929PurposeaTc inducible HypaCas9DepositorInsertHypaCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698APromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9-D1135E
Plasmid#154930PurposeaTc inducible HypaCas9 with D1135E mutationDepositorInsertHypaCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698A/D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9-D1135E
Plasmid#154931PurposeaTc inducible Cas9 with D1135E mutationDepositorInsertCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationD1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0296
Plasmid#162305PurposeLevel 0 CDS part for MoClo assembly, AATG-TTCGDepositorInsertCDS, superfolder GFP, no stop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0030
Plasmid#162312PurposeLevel 0 C-terminal tag part for MoClo assembly, TTCG-GCTTDepositorInsertC-terminal tag, stop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0549
Plasmid#162300PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, SUMO-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPYC0CM0258
Plasmid#162291PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNK131d
Plasmid#156369PurposeExpresses heterlogous ribosomal proteinsDepositorInsertS20, S16, S1, S15 ribosomal genes
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB184h
Plasmid#156365PurposeExpresses heterlogous ribosomal proteinsDepositorInsertS20, S16, S1, S15 ribosomal genes
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB184g
Plasmid#156364PurposeExpresses heterlogous ribosomal proteinsDepositorInsertS20, S16, S1, S15 ribosomal genes
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only