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Plasmid#180718PurposeSpacer cassette with ConL4 and ConR5 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert4/5 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT064
Plasmid#180717PurposeSpacer cassette with ConL4 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert4/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT063
Plasmid#180716PurposeSpacer cassette with ConL3 and ConR4 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert3/4 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT062
Plasmid#180715PurposeSpacer cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert3/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT061
Plasmid#180714PurposeSpacer cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert2/3 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT060
Plasmid#180713PurposeSpacer cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert2/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT059
Plasmid#180712PurposeSpacer cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert1/2 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT058
Plasmid#180711PurposeSpacer cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert1/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT057
Plasmid#180710PurposeSpacer cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertS/1 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA position E2 (GB2239)
Plasmid#160561PurposetRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNADepositorInserttRNA-gRNA position E2 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EBFP2(1-10)
Plasmid#165141PurposeExpresses EBFP2(1-10) in mammalian cellsDepositorInsertEBFP2(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mRuby4(1-10)
Plasmid#165143PurposeExpresses mRuby4(1-10) in mammalian cellsDepositorInsertmRuby4(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDuex2-rsEGFP2-E
Plasmid#205753PurposeExpression of rsEGFP2-E in mamalian cellsDepositorInsertrsEGFP2-E
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only