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Plasmid#91895PurposesgNf1.1 for Nf1 deletionDepositorInsertsgNf1.1
UseLentiviral and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2MT-mbeta-catenin delta2
Plasmid#100879PurposeMammalian expression of mouse beta-catenin truncationDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseTags6xMycExpressionMammalianMutationtruncated Ctnnb1 1-276aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2MT-mbeta-catenin delta1
Plasmid#100878PurposeMammalian expression of mouse beta-catenin truncationDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseTags6xMycExpressionMammalianMutationtruncated Ctnnb1 1-150aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-mCherry
Plasmid#72896PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherryDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2.sgNf1.4
Plasmid#92029PurposesgNf1.4 for Nf1 deletionDepositorInsertsgNf1.4 for Nf1 deletion
UseLentiviral and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2VL-IRES-dOrai-IRES-GFP
Plasmid#72897PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2VL-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorInsertOrco (Y478A) (Orco Fly)
UseUnspecifiedTagsExpressionMutationchanged 478 Tyrosine for AlaninePromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.hOr47a
Plasmid#126474PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsV5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Donor_C_FH_VIR
Plasmid#186660PurposeDonor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Shu
Plasmid#186655PurposeDonor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_C_FH_Nbr
Plasmid#186662PurposeDonor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.DepositorInsertOSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_N_FH_GLE
Plasmid#186657PurposeDonor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-mTert
Plasmid#162555PurposeOverexpression of TertDepositorInsertOverexpression of Tert (Tert Mouse)
UseTagsExpressionMammalianMutationNonePromoterEF1AAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
Plasmid#58959Purposelentiviral expression of v-H-Ras and luciferase/EGFP reporterDepositorInsertsCMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene
UseLentiviralTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-HA-Jag1
Plasmid#46052DepositorInsertJag1 (Jag1 Rat)
UseEntry vectorTagsHA tagExpressionMutationPromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.mER.hOr47a
Plasmid#126473PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsH. sapiens HCN1 106VNKFSL111 (mER) and V5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
Plasmid#128278PurposeExpresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell cultureDepositorInserttwinstar (tsr) (tsr Fly)
UseTags6X Histidine tagExpressionBacterialMutationMet1 changed to Pro1; Cys77 silent basepair mutat…PromoterT7Available sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Piwi
Plasmid#186651PurposeDonor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only