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Plasmid#107886PurposeR4 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-151TGA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT004b
Plasmid#107884PurposeR2 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-151TGA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-PEAR-mCherry
Plasmid#228892PurposeAll-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.DepositorInsertEGFP-IRES-mScarlet
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD
Plasmid#133300PurposeThe puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWT004j
Plasmid#107887PurposeR5 plasmid. Contains PBAD-EGFP-115-135insertion, T206G and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-115-135insertion, T206G
UseTagsExpressionBacterialMutationinsertion of nt 115-135, T206G nt changePromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDT305
Plasmid#128801PurposeHuman codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backboneDepositorInsertdCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
UseCRISPR and LentiviralTagsExpressionMutationPromoterhUBC promoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
UseTagsExpressionYeastMutationPromoterGAL1 promoter and hybrid dual-mode promoter-see c…Available sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pERB215
Plasmid#61499PurposeConst. Halotag-GFP-Nuf2 + mCherry-DHFRDepositorInsertsHaloTag-GFP-Nuf2
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB221
Plasmid#61502Purposeconst. CenpB-GFP-Halo + mCherry-DHFRDepositorInsertsCenpB-GFP-Halo
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB217
Plasmid#61500PurposeConst. Halotag-GFP-Mito + mCherry-DHFRDepositorInsertsHaloTag-GFP-Mito
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB219
Plasmid#61501PurposeConst. Halotag-GFP-PACT + mCherry-DHFRDepositorInsertsHaloTag-GFP-PACT
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHu6-gRNA-NT1
Plasmid#81203PurposehU6 expression of gRNA NT1DepositorInserthU6 expression of gRNA NT1
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSD080A
Plasmid#218138PurposeLentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.DepositorInsertSD40-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD141B
Plasmid#218139PurposeLentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.DepositorInsertSD56-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD068F
Plasmid#218137PurposeLentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.DepositorInsertSD36-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCROPseq-VEX-optimized-scaffold
Plasmid#203321PurposeModified CROPseq vector for efficient CRISPR screens in hematopoiesisDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-LD)
Plasmid#214420PurposeDetection of ER-lipid droplet contact siteDepositorInsertRspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(mito-LD)
Plasmid#214421PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mApple-6xHp
Plasmid#217376PurposeLipid droplet localization of 6xHp of Spastin fused to mAppleDepositorInsertmApple-6x Hp NC
UseLentiviralTagsmAppleExpressionMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-mito)
Plasmid#214423PurposeDetection of ER-mito contact siteDepositorInsertRspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
UseLentiviralTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only