...biotechnology products Dr. Idah Sithole-Niang, University of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang ... cassava mosaic virus Dr. Fiona Robertson, University of Zimbabwe, Zimbabwe Dr. Fiona Robertson (far ...source of dietary supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate... detection Dr. Rodrigo Arturo Rivera Solís, Universidad Autónoma de Yucatán, Mexico Dr. Rodrigo Arturo...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...increase the potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology...

...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...Dr. Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a ... CRISPR Plasmids Dr. Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab...

...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...questions. To keep up with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow...

...blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...ATP or other chemical fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different...

...either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set...ADH1 terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple...plasmids for genome modification in yeast developed by Dr. Pringle’s former lab at UNC Chapel Hill. Bähler ...

...includes a set of plasmids each containing an anti-GFP nanobody fused with a different functional element...using different detection methods. Since the anti-GFP nanobody is able to bind fluorescent proteins derived...here can be used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores. Find...to the MarathonRT podcast segment Researchers from Dr. Anna Pyle’s lab at Yale University recently deposited...

...engineered BFP variant that converts to GFP after a base editing event. GFP positive cells can be sorted and ... their hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains...the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...lab was inspired by work that used an integrated GFP reporter that is converted into BFP (blue fluorescent...engineered a BFP variant that undergoes conversion to GFP after being targeted by a cytidine deaminase base... a successful base editing event would result in GFP fluorescence that can be visualized and sorted via...results in shift in emission spectra from BFP to GFP. Image from Standage-Beier et al., 2019. The team...

...electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes...with autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation...autophagosomes and autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog...pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). They are ...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring...

...technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase...Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning and...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...#17408)   This is a lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin...

...ends up being translational. Ex: the discovery of GFP. Chapter 4: Goals of the Center for Regenerative ...place?" Many thanks to our guest, Darrell Kotton. Dr. Kotton is the David C. Seldin Professor in the Department...

...pPS808 - N-terminal GFP for yeast expression pET GFP - C-terminal GFP for...gene expression GFP Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral ...C elegans expression GFP Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... from the SP6 promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription ...expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian expression...expression MSCV-IRES-GFP - Mammalian retroviral gene expression with GFP selectable ...cassette pBTG (pBigT-IRES-GFP) - pBigT modified to contain IRES-GFP Hynes Lab ...

...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic ...Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working to develop...

...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...recombination by finding acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs...This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions,...fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...

...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...upstream of the final ITR and downstream of the WPRE. TL;DR? Watch our AAV Transfer Plasmid video!   Now you...

...recently deposited with Addgene by Dr. Mark Prescott. Dr. Prescott and Dr. Rod Devenish (both at Monash University..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are now providing... 8,158 predicted T. gondii protein-coding genes). Dr. Lourido’s studies demonstrate that genome-scale ...

...dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP, and...and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown...step for CRISPR/Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists ...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on...and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the...enabled scientists to engineer a wide variety of GFPs with diversity in fluorescence brightness, intensity...plasmid undergoes HR or SSA to reconstitute functional GFP, and the cells will turn green. Mashiko et al.,...

...containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA...viral titre to the number of cells transduced by a GFP encoded virus, and the reverse transcriptase activity...Regulatable Lentiviral Vector System, Viral Gene Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4....

...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate...Hennessey E, Butcher R, Koblan LW, Comander J, Liu Q, Liu DR (2020) Cytosine and adenine base editing of the brain...

...Cressey is a PhD Student in the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene...

...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP...GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A GFP-Rab7A     ...protein Knock-off, is made up of three components: a GFP protein that binds to the coding region of a reporter...reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter...these dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize...monoclonal antibodies against them, fused them to GFP, and compared them to popularly used epitopes tags...