Showing: 261 - 280 of 4601 results
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Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailabilityAcademic Institutions and Nonprofits only -
pMLM3705
Plasmid#47754PurposeExpresses mammalian cell codon-optimized dCas9-VP64DepositorInsertcodon optimized dCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9 (catalytically i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
UseTags6XHisExpressionYeastMutationPlease see depositor commentsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mTurquoise2
Plasmid#108235PurposeBroad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPc promoter - mTurquoise2
UseTagsExpressionBacterialMutationcodon optimized for experession in P. fluorescensPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag-Pcbp2
Plasmid#184028PurposeCodon optimized Pcbp2 clone with N-terminal flag tagDepositorInsertPcbp2 (Pcbp2 Mouse)
UseTagsFlagExpressionMammalianMutationCodon optimizedPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-Halo
Plasmid#184417Purposevector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsHalo-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2+/C-Halo
Plasmid#184418Purposevector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsHalo-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailabilityAcademic Institutions and Nonprofits only -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)
Plasmid#185915PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
R777-E111 Hs.IRS2
Plasmid#70395PurposeGateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)DepositorInsertIRS2 (IRS2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 261 - 280 of 4601 results