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Plasmid#191207PurposetdTomato expression under the control of TRE promotorDepositorInsertTdTomato
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)dTomato
Plasmid#200113PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Lck-tdTomato
Plasmid#118739PurposeTo express membrane targeted tdTomatoDepositorInsertLck domain (LCK Human)
UseLentiviralTagstdTomatoExpressionMutationH8N (see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:GtACR1-tdTomato
Plasmid#124235PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomatoDepositorInsertGtACR1-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChIEF-tdTomato
Plasmid#50977PurposeAAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITRDepositorInsertChIEF-tdTomato
UseAAVTagstdTomatoExpressionMutationChIEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E)dTomato
Plasmid#200110PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element, no other promoter elemen…Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAmetrine-tdTomato-FRET
Plasmid#58165PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmAmetrine and tdTomatoExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Septin 2 -tdTomato
Plasmid#118735PurposeTo express Septin 2 tagged with tdTomato on C-terminusDepositorInsertSeptin 2 (SEPTIN2 Human)
UseLentiviralTagstdTomatoExpressionMutationPromoterCMVAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Integrin-Beta1-N-18
Plasmid#58105PurposeLocalization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581DepositorInsertIntegrin-Beta1 (ITGB1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
Plasmid#85453PurposeLentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.DepositorInsertU6-sgRNA-Backbone-UP-TdTomato
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationdeletion of AA123-365 in tdTomato (please see dep…PromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-C6F/TdTomato
Plasmid#140826PurposeOCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)DepositorInsertsUseTagsExpressionMammalianMutationL104P- please see depositor commentsPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-terminator (SL695)
Plasmid#133961PurposepUC19 plasmid with U6 promoter followed by a poly-T termination signalDepositorInsertU6 promoter
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-PLIN2-tdTOMATO
Plasmid#172533PurposePLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloningDepositorInsertplin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F, and R415H mutations in …PromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-N–dSpCas9
Plasmid#157835Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertbpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-tdTomato
Plasmid#122101PurposeAAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInserttdTomato
UseAAVTagsNAExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Beta-Catenin-20
Plasmid#58071PurposeLocalization: Tight Junctions, Excitation: 554, Emission: 581DepositorInsertBeta-Catenin (Ctnnb1 Mouse)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBa.PEX3-tdTomato-FKBP
Plasmid#193941PurposeMammalian expression of tdTomato-tagged PEX3DepositorInsertPEX3 (PEX3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-tdTomato
Plasmid#62516PurposeExpresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing proceduresDepositorInserttdTomato
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS TdTomato IX
Plasmid#37347DepositorInsertNLS Tdtomato
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only