Showing: 261 - 280 of 868 results
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Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMOD_A-AtUBI10-dCas9-24XGP41
Plasmid#202011PurposeMoclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSPDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo level 1 vectorTagsExpressionMutationPromoterAtUBI10AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSc1-puro
Plasmid#80438PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycinDepositorInsertssp Cas9 gRNA
eGFP
Puromycin resistance
UseCRISPRTagsExpressionMammalianMutationPromoterCMV, U6, and hPGKAvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
Eef1a-1955/-1>nls::Cas9::nls
Plasmid#59987PurposeEef1a (EF1alpha) promoter driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Eef1a (EF1alpha) -1955/-1AvailabilityAcademic Institutions and Nonprofits only -
Mesp-1916/-1>nls::Cas9::nls
Plasmid#59988PurposeMesp driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Mesp -1916/-1AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CibN-dCas9
Plasmid#60551PurposeExpresses CibN-dCas9 in mammalian cellsDepositorInsertCibN-dCas9
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAE03
Plasmid#61044PurposeTo construct C-terminal GFP fusions, single crossover integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJWV25
Plasmid#61045PurposeTo construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaADepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMJ825
Plasmid#39315DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MLM3613
Plasmid#42251PurposeExpresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promotersDepositorInsertStreptococcus pyogenes Cas9
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKB01_mCherry
Plasmid#69104PurposeExpresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationR691A from wild-type Cas9PromoterT3AvailabilityAcademic Institutions and Nonprofits only -
pFD115
Plasmid#124768PurposepFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
cas9
term PpflB sgRNA (BsaI)
UseCRISPRTagsExpressionMutationPromoterPtetAvailabilityAcademic Institutions and Nonprofits only
Showing: 261 - 280 of 868 results