Addgene

Showing: 261 - 280 of 542 results

Results per page:

Sort results by:

pEGFP.N3-SLC35A2-GFP

Plasmid

#186284

Purpose

transient expression of C-terminal GFP tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP.N3-SLC35A1-GFP

Plasmid

#186281

Purpose

transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3B no-stop

Plasmid

#123205

Purpose

Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3A no-stop

Plasmid

#123204

Purpose

Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-SLC35B2-sgRNA

Plasmid

#154860

Purpose

Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 sgRNA (SLC35B2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3A G120A

Plasmid

#123070

Purpose

Gateway entry clone encoding human MAP1LC3A G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP/Venus-rLC3

Plasmid

#58740

Purpose

Expresses Venus-rLC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3B G120A

Plasmid

#123073

Purpose

Gateway entry clone encoding human MAP1LC3B G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX- HcRed -hLC3ΔG

Plasmid

#24992

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)

Use

Tags

HcRed

Expression

Mammalian

Mutation

deleted Gly at 121

Promoter

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3C no-stop

Plasmid

#123208

Purpose

Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-LC3-K51A

Plasmid

#155265

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Lysine 51 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3C G126

Plasmid

#123079

Purpose

Gateway entry clone encoding human MAP1LC3C G126

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX-YFP-hLC3ΔG

Plasmid

#24990

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with EYFP (MAP1LC3B Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

deleted Gly at 121

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-3×FLAG-SLC35A1

Plasmid

#186282

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Use

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SLC30A10-E25A

Plasmid

#82345

Purpose

Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

E25A

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge