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PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

Plasmid

#158542

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion, F188D, W2…

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 110-480 WT

Plasmid

#158543

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 110-480, C-terminal deletion

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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HR700_HA2TP53_Ex5

Plasmid

#228860

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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HR700_HA2TP53_Ex6

Plasmid

#228861

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HR700_HA2TP53_Ex7

Plasmid

#228862

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HR700_HA2TP53_Ex8

Plasmid

#228863

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CuO-V5-CASC3 siRes 110-480 188/218-mut

Plasmid

#158544

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 110-480, C-terminal deletion, F188D, …

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1A

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-2xFYVE

Plasmid

#136996

Purpose

Lentiviral EGFP lipid sensor for PI(3)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-2xFYVE

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-P4M-SidMx2

Plasmid

#136997

Purpose

Lentiviral EGFP lipid sensor for PI(4)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-P4M-SidMx2

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PH-PLC-delta-EGFP

Plasmid

#136998

Purpose

Lentiviral EGFP lipid sensor for PI(4,5)P2

Depositor

Ken-Ichi Takemaru

Insert

PH-PLC-delta-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-mSstr3-EGFP

Plasmid

#200444

Purpose

Lentiviral expression of mouse Sstr3-EGFP

Depositor

Ken-Ichi Takemaru

Insert

Sstr3 (Sstr3 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EB1-EGFP

Plasmid

#118084

Purpose

Expresses EB1-EGFP in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

human EB1 (MAPRE1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-IFT20-EGFP

Plasmid

#118032

Purpose

Expresses IFT20-EGFP in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

human IFT20 (IFT20 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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IRES-EGFP-Flag-hRabin8

Plasmid

#118073

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

human Rabin8 (RAB3IP Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-mIFT88-EGFP

Plasmid

#149697

Purpose

Expresses mouse IFT88-EGFP in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

IFT88 (Ift88 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-hChibby1

Plasmid

#89770

Purpose

Labels mother centrioles/basal bodies

Depositor

Ken-Ichi Takemaru

Insert

Chibby1 (CBY1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1alpha

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-Rabin8

Plasmid

#118072

Purpose

Expresses EGFP-Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

human Rabin8 (CBY1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-T2-CeNLuc

Plasmid

#135948

Purpose

Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-CeNLuc

Plasmid

#135952

Purpose

Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Use

Tags

2xSV40-NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge