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Mtp-p53-ECFP

Plasmid

#53961

Purpose

Expresses CFP tagged p53 in mammalian cells

Depositor

Galit Lahav, Uri Alon

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

p53 (TP53 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

metallothionein promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Use

Tags

ECFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAM-CFP

Plasmid

#13027

Depositor

Doug Golenbock

Insert

Toll-like Receptor Adaptor Molecule (TICAM2 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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cyto-CFP-FRBx5

Plasmid

#103776

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-CFP-FRBx5

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pME-nfsB-CFP

Plasmid

#86374

Purpose

expression of nfsB-CFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase

Use

Zebrafish

Tags

ECFP

Expression

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-SECFP-Galectin3

Plasmid

#64150

Purpose

Expression in mammalian cells (production of retrovirus vector)

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Use

Retroviral

Tags

SECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
Vimentin - APEX2 in pECFP

Plasmid

#66170

Purpose

APEX2 fused to the C-terminus human vimentin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

human Vimentin - APEX2 (VIM Human, Synthetic)

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pARF1(Q71L)-CFP

Plasmid

#11385

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Changed Glutamine 71 to Leucine.

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-SECFP

Plasmid

#175838

Purpose

Express SECFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pARF1(T31N)-CFP

Plasmid

#11384

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 31 to Asparagine.

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR6-CFP

Plasmid

#13021

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 6 (TLR6 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p2R3a-Tq2CFP-OcsT

Plasmid

#71268

Purpose

Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Tq2CFP

Use

Gateway

Tags

4xGly linker and octaline synthase terminator

Expression

Mutation

Promoter

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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G protein alpha-q-CFP

Plasmid

#66081

Purpose

This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

Depositor

Catherine Berlot

Article

Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

Insert

G protein alpha-q-EE-CFP (Gnaq Mouse)

Use

Tags

CFP was inserted internally into the alpha-q sequ…

Expression

Mammalian

Mutation

BamHI and SacI sites in alpha-q were removed with…

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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ps-3HA-Beta2-PSCFP2-N

Plasmid

#114186

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Use

Tags

3HA and PS-CFP2

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pECFP-DEVG-Venus

Plasmid

#24538

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVG-Venus

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

DEVDR mutated to DEVG

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only