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pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
UseTagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Tyr-P(11)4
Plasmid#185784PurposeEncodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTyr-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT43_GalL_BE-P(AP)3
Plasmid#145036PurposeExpresses BE-P(AP)3 in yeast cellsDepositorInsertBE-(AP)3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSP6
Plasmid#48150PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB035a
Plasmid#79190PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT45_GalL_nCDA1-BE3
Plasmid#145038PurposeExpresses nCDA1-BE3 in yeast cellsDepositorInsertnCDA1-BE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB029f
Plasmid#79220PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-SH2ABL1; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB031a
Plasmid#79221PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ HA4-Zif268; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT79_GalL_nCDA1Δ192-BE3
Plasmid#145045PurposeExpresses nCDA1Δ192-BE3 in yeast cellsDepositorInsertnCDA1Δ192-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-192AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB043c
Plasmid#79223PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-p38-alpha; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-NaChBac
Plasmid#125580PurposeCre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.DepositorInsertNaChBac
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB042b
Plasmid#79193PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-MBPoff7
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB042d
Plasmid#79194PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-p38_2_3
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB043a
Plasmid#79222PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-MBP; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceApril 15, 2017AvailabilityAcademic Institutions and Nonprofits only
