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Showing: 391 - 420 of 493 results

  1. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

    Plasmid
    #201187
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 2 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201187-plasmid-map-sequence-id-398106

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  2. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

    Plasmid
    #201188
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 3 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201188-plasmid-map-sequence-id-398098

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  4. Arch(D95H)-eGFP

    Plasmid
    #51081
    Purpose
    Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Histidine
    Promoter
    Ubiquitin
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51081-plasmid-map-sequence-id-77349

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  5. pLV-SFFV-RTN3-WPRE-UbC-Emerald

    Plasmid
    #233467
    Purpose
    Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    RTN3 (RTN3 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    233467-plasmid-map-sequence-id-465704

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  7. pDG02583

    Plasmid
    #104129
    Purpose
    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    NEDD8-specific protease 1 (NEDP1)
    Use
    Tags
    14x Histidine tag, Maltose binding protein, and S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104129-plasmid-map-sequence-id-313426

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  11. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid
    #214468
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214468-plasmid-map-sequence-id-424065

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  12. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid
    #214471
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214471-plasmid-map-sequence-id-425103

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  13. PSL1180polyUBdsRED

    Plasmid
    #49327
    Purpose
    Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    dsRED
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes polyubiquitin
    Available since
    Nov. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49327-plasmid-map-sequence-id-73022

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  14. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Use
    Tags
    NLS
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

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  15. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

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  16. pRGE32

    Plasmid
    #63159
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63159-plasmid-map-sequence-id-105873

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  20. pLVUT-eC3GFP

    Plasmid
    #225485
    Purpose
    Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP
    Depositor
    Robert Gross
    Article
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251, See Depositor Comments
    Promoter
    Human ubiquitin C
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225485-plasmid-map-sequence-id-444523

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  23. pOD1988-epiDEG

    Plasmid
    #89357
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pdpy-7
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89357-plasmid-map-sequence-id-177228

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  24. pOD2046-bwmDEG

    Plasmid
    #89367
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmyo-3
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89367-plasmid-map-sequence-id-177233

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  25. pOD2044-intDEG

    Plasmid
    #89366
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pelt-2
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89366-plasmid-map-sequence-id-177231

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  27. pYPQ141-ZmUbi-RZ-As

    Plasmid
    #86196
    Purpose
    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Insert
    AsCpf1 gRNA cloning site for ribozyme cleavage
    Use
    CRISPR
    Tags
    Hammerhead ribozyme and HDV ribozyme
    Expression
    Plant
    Mutation
    Promoter
    Maize ubiquitin 1
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    86196-plasmid-map-sequence-id-176061

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  28. pOD2048-csnDEG

    Plasmid
    #89368
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Posm-6
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  29. pOD2087-trnDEG

    Plasmid
    #89369
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmec-18
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89369-plasmid-map-sequence-id-177235

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  30. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Available since
    Aug. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

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Showing: 391 - 420 of 493 results