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pCS2-Bighead S-FC

Plasmid

#180147

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

Tags

FC

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+WBP2NL-HA

Plasmid

#174791

Purpose

Xenopus laevis WBP2NL with 3'HA tag

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

WBP2 N-terminal like (wbp2nl.L Frog)

Use

Tags

HA

Expression

Bacterial

Mutation

aa#165, Y to H; aa# 287, D to H

Promoter

SP6

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlT45A

Plasmid

#172464

Purpose

expresses protein with one aa substitution to affect potential phosphorylation

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlT45A

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Threonine at aa45 to Alanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2xVlk1-3xFlag

Plasmid

#172773

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

Insert

Vlk1 (pkdcc.1.S Frog)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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KSP_HNL

Plasmid

#129772

Purpose

Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

Depositor

William Hancock

Insert

KSP (kif11.L Frog)

Use

Tags

His6

Expression

Bacterial

Mutation

Truncation at 367

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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p64T-XBMP2

Plasmid

#117253

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP2

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-XTbx5

Plasmid

#117247

Purpose

mRNA synthesis in situ hyb probes

Depositor

Gerald Thomsen

Article

Horb et al Development. 1999 Apr;126(8):1739-51.

Insert

Tbx5 (tbx5-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-BRAT

Plasmid

#117251

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1997 May;124(9):1689-98.

Insert

BRAT (vegt-a Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1C699A

Plasmid

#117245

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1-C699A mutant

Use

Xenopus expression

Tags

Expression

Mutation

mutated cysteine 699 to alanine

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1

Plasmid

#117244

Purpose

mRNA synthesis in situ hyb probes

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-BRAT-EnR

Plasmid

#117252

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1997 May;124(9):1689-98.

Insert

BRAT-EnR (vegt-a Frog)

Use

Xenopus expression

Tags

Expression

Mutation

chimeric protein with engrailed repressor domain…

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSP64T-BMP4

Plasmid

#117254

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP4

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-XTbx5-EnR

Plasmid

#117249

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1999 Apr;126(8):1739-51.

Insert

Tbx5-EnR (tbx5-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

chimeric protein with engrailed repressor domain…

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge