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Plasmid#144514PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIRF2 (IRF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1568
Plasmid#143962PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF683 (ZNF683 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab8A
Plasmid#166428PurposeRab8A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab8A
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-3
Plasmid#71722Purposetyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorUseTagsExpressionMutationPromoterpTrcAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab10
Plasmid#166429PurposeRab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab10
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC23A1
Plasmid#132013PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC23A1 (SLC23A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET26b-Granzyme A (S-A)
Plasmid#8825DepositorInsertHuman Granzyme A inactive (GZMA Human)
UseTags(His)6ExpressionBacterialMutationchanged Serine 184 to AlaninePromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC5A12_STOP
Plasmid#161395PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC5A12 (SLC5A12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Myc-XPLN
Plasmid#73365PurposeExpresses myc tagged XPLN in mammalian cellsDepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV-AICD
Plasmid#69927Purposebacterial expression of MBP-TEV-AICDDepositorInsertAICD (APP Human)
UseTagsMBP-TEVExpressionBacterialMutation47 aa longPromoterT7Available sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag PA1
Plasmid#15560DepositorInsertPA1 (PAGR1 Human)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
mVenus-Vinculin-23
Plasmid#56625PurposeLocalization: Focal Adhesions, Excitation: 515, Emission: 528DepositorInsertVinculin (VCL Human)
UseTagsmVenusExpressionMammalianMutationV234L in VinculinPromoterCMVAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TIS11B CPM2
Plasmid#121143PurposeMammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)DepositorInsertZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD86
Plasmid#223598PurposeLentiviral expression of human CD86 with SpyTag003 in mammalian cellsDepositorInsertCD86 (CD86 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-R84S
Plasmid#213591PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84SPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-FFSS-Cyclin D1
Plasmid#215146PurposeExpresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagsFFSS (FLAG-FLAG-STREP-STREP)ExpressionMammalianMutationPromoterTRE3GSAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC28A1_STOP
Plasmid#161246PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC28A1 (SLC28A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_GNA13_p.L374I
Plasmid#81350PurposeGateway Donor vector containing GNA13 , part of the Target Accelerator Plasmid Collection.DepositorInsertGNA13 (GNA13 Human)
UseGateway entry vectorTagsExpressionMutationL374IPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only