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Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHJL2
Plasmid#199377PurposeEncodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6f::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH2217
Plasmid#179251PurposePsra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherryDepositorInsertTWK-18 (twk-18 Nematode)
UseTagswCherryExpressionWormMutationM280IPromoterPsra-11Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxTagsExpressionWormMutationHYGR∆ encodes aa 1-226PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-RGG
Plasmid#124946PurposeExpression of MBP-RGG-RGGDepositorInsertMBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
Plasmid#159811PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD04neo pmyo2::gfp
Plasmid#26393DepositorTypeEmpty backboneUseTagsExpressionBacterial and WormMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDD362
Plasmid#137023PurposeSapTrap HaloTag-GLO donorDepositorInsertC. elegans GLO-optimized Halo Tag
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized Halo TagExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr
Plasmid#19326DepositorArticleInsertPmyo-3::twk-18(cn110)cDNA
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEY22
Plasmid#191028Purposelim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertlim-4 (lim-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY17
Plasmid#191023Purposeflp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-18 CELE_Y48D7A.2 (flp-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY16
Plasmid#191022Purposeflp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-6 (flp-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY18
Plasmid#191024Purposeflp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-19 (flp-19 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY19
Plasmid#191025Purposeflp-26 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-26 (flp-26 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY21
Plasmid#191027Purposeggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertggr-3
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY23
Plasmid#191029Purposepdfr-1(2-1k) fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertpdfr-1(2-1k) (pdfr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY24
Plasmid#191030Purposesrab-20 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertsrab-20 (srab-20 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY27
Plasmid#191032Purposeflp-13 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertflp-13 (flp-13 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY28
Plasmid#191033Purposeflp-20 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertflp-20 (flp-20 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY29
Plasmid#191034Purposegcy-36 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-36 (gcy-36 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only