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Showing: 261 - 280 of 3934 results

  2. mCherry2-C1

    Plasmid
    #54563
    Purpose
    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54563-plasmid-map-sequence-id-310151

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  8. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  10. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid
    #127289
    Purpose
    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
    Depositor
    Fred Gage
    Article
    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1
    Inserts
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Ngn2 and Ascl1 linked via 2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127289-plasmid-map-sequence-id-308425

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  11. pDG02583

    Plasmid
    #104129
    Purpose
    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    NEDD8-specific protease 1 (NEDP1)
    Use
    Tags
    14x Histidine tag, Maltose binding protein, and S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104129-plasmid-map-sequence-id-313426

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  13. mEGFP-C1

    Plasmid
    #54759
    Purpose
    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54759-plasmid-map-sequence-id-320190

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  14. pRL443

    Plasmid
    #70261
    Purpose
    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70261-plasmid-map-sequence-id-348706

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  18. Lamp1-mScarlet-I

    Plasmid
    #98827
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98827-plasmid-map-sequence-id-430619

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  19. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  20. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-112648

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Showing: 261 - 280 of 3934 results