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pFA6a-GBP-kanMX6

Plasmid

#89067

Purpose

genomic GBP epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP

Expression

Yeast

Mutation

Promoter

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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SIVl

Plasmid

#81090

Purpose

Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSH100 (YCplac33 MET25pro MCP-mCherry)

Plasmid

#45930

Depositor

Daniel Zenklusen, Robert Singer

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

MCP-mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Met25

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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SIVt

Plasmid

#81092

Purpose

Single Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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SIVh

Plasmid

#81091

Purpose

Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEGFP-KanMX6

Plasmid

#87023

Purpose

Tag S. pombe genes in C terminal with mEGFP

Depositor

Julien Berro

Article

Akamatsu et al J Cell Biol. 2014 Mar 17;204(6):977-88.

Insert

mEGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBF3060

Plasmid

#26853

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2010 Sep 10. ():.

Insert

CreA recombinase

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

N/A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pI6-MTU-DO-URA

Plasmid

#160030

Purpose

Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HPH

Plasmid

#160216

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-URA

Plasmid

#160015

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pH3F-GAL1

Plasmid

#85775

Purpose

N-ICE plasmid pH3F-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pH3FS

Plasmid

#85780

Purpose

N-ICE plasmid pH3FS

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBF3038

Plasmid

#26850

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2010 Sep 10. ():.

Insert

CreA recombinase

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

N/A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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RS314-SN

Plasmid

#17539

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pK3FS

Plasmid

#85776

Purpose

N-ICE plasmid pK3FS

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-nLuc

Plasmid

#199756

Purpose

Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHES822

Plasmid

#89193

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pZ

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pJYDNgp

Plasmid

#162453

Purpose

Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-2Glinker-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

HA nad myc and eUnaG2 fluorescent protein

Expression

Mutation

Promoter

GAL1

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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