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Plasmid#134857PurposeExpresses EGFP-tagged synaptic vesicle markerDepositorInsertRas-related protein 3C
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianMutationPromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) PHLDB1
Plasmid#107503PurposeExpresses PHLDB1, puromycin resistantDepositorInsertPHLDB1 (PHLDB1 Human)
UseLentiviralTagsExpressionMammalianMutationNucleotide changes 1236 T>C, 1365 C>T, 1371…PromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA
Plasmid#204547PurposeExpresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cellsDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsExpressionMammalianMutationdeltaUBAPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2
Plasmid#117406PurposeSpyCas9 sgRNA 2 targeting TLR2.0DepositorInsertSpyCas9 sgRNA targeting TLR 2.0
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA
Plasmid#117411PurposeAsCas12a-gRNA targeting TLR2.0DepositorInsertAsCas12a gRNA targeting TLR 2.0
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor
Plasmid#86843PurposeHR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.DepositorInsertFLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
UseTagsFLAG-HaloTagExpressionMammalianMutationPromoterEndogenous TERT promoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)
Plasmid#117134PurposeAll-in-one expression vector for Cas9 and gRNA against CD44DepositorInsertgCD44 v2 (Cd44 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro METTL3
Plasmid#200724PurposeLentiviral expression of Flag-Tagged human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro NFIL3 P2A eGFP NLS
Plasmid#140026PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A TagRFP-T P2A puro
Plasmid#122200PurposeThe plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsspCas9
TagRFP-T
UseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR
Plasmid#194925PurposemRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected hoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterhPGKAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)
Plasmid#139446PurposeLentiviral vector genome to ectopically express Firefly luciferaseDepositorInsertFLAG-tag Firefly luciferase
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin
Plasmid#134869PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcytochrome b5 type A (CYB5A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-98PromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationdeleted bromodomainPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
stblHIF: pLenti-PGK-stblHIF-PGK-Puro
Plasmid#177202Purposeencoding stblHIF under PGK promoterDepositorArticleInsertstblHIF
UseLentiviralTagsExpressionMammalianMutationP402A/P577G/N813APromoterPGKAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A mNeonGreen P2A puro
Plasmid#122183PurposeThe plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only