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Plasmid#112655PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K Δ2x+ve
UseTagsExpressionYeastMutationR59K, mutations (R223E, K224E, K225E, K233E, K234…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
UseTagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA16
Plasmid#158494PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, D481V, no STOP codonPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA7
Plasmid#158485PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA15
Plasmid#158493PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA6
Plasmid#158484PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationD481V, STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA14
Plasmid#158492PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationD481V, no STOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorInsertMYO7B sgRNA (MYO7B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A-SRDX
Plasmid#129683PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b-SRDX
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD573A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS
Plasmid#121827PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-Cas9 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY001: pMVP (L3-L2) BioID2 + polyA
Plasmid#121795PurposepMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.DepositorInsertBioID2 + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY202: pMVP (L3-L2) APEX2 + polyA
Plasmid#121796PurposepMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.DepositorInsertAPEX2 + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JD602: pMVP (L3-L2) APEX2 + WPRE
Plasmid#121798PurposepMVP L3-L2 entry plasmid, contains APEX2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest in lentivirus vectors.DepositorInsertAPEX2 + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC601: pMVP (L3-L2) eYFP + polyA
Plasmid#121769PurposepMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.DepositorInserteYFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC801: pMVP (L3-L2) eGFP + WPRE
Plasmid#121771PurposepMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.DepositorInserteGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC901: pMVP (L3-L2) mCherry + WPRE
Plasmid#121773PurposepMVP L3-L2 entry plasmid, contains mCherry + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest in lentivirus vectors.DepositorInsertmCherry + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD001: pMVP (L3-L2) eYFP + WPRE
Plasmid#121777PurposepMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.DepositorInserteYFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG901: pMVP (L3-L2) HA epitope tag + polyA
Plasmid#121749PurposepMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertHA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only