Addgene

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YFP-Gamma 9-3

Plasmid

#36074

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein G9(gamma 3 tail)

Use

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (D14A/G38A)

Plasmid

#128118

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (D14A/G38A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-2xMARS-PLD

Plasmid

#205237

Purpose

Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

Depositor

Jeremy Baskin

Inserts

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
phospholipase D (PLD)

Use

Tags

Nuclear Export Sequence and mCherry

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-LOVPLD-mCherry-OSBP(PH)

Plasmid

#219561

Purpose

Golgi-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

LOVPLD-mCherry-OSBP(PH)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-lyn10-mCherry-dLOVPLD

Plasmid

#219556

Purpose

PM-targeted catalytically dead LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-mCherry-dLOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

H170A mutation on PLD

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-p18-mCherry-LOVPLD

Plasmid

#219560

Purpose

lysosome-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

p18-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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HiBiT-SMO

Plasmid

#195851

Purpose

Expresses mouse SMO with an N-terminal HiBiT; contains D54N and R690G

Depositor

Gunnar Schulte

Article

Turku et al Nat Commun. 2021 Jun 24;12(1):3919. doi:

Insert

SMO (Smo Mouse)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

D54N and R690G

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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V5-K202R-short-UBASH3A

Plasmid

#192084

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

K202R

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mNTERY07

Plasmid

#184117

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY07

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mNTERY06

Plasmid

#184116

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY06

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mNTERY05

Plasmid

#184115

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY05

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mNTERY04

Plasmid

#184114

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY04

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mNTERY02

Plasmid

#184112

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY02

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mNTERY08

Plasmid

#184118

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY08

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mNTERY01

Plasmid

#184111

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY01

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lmajor_gp63_28_0570_WT

Plasmid

#171642

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.28.0570

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_31_2000_WT

Plasmid

#171643

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 31 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.31.2000

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPLuc-E2A-cyOFP1

Plasmid

#141084

Purpose

precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-E2A-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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PA-Bongwoori-R3

Plasmid

#137838

Purpose

A photoactivatable GEPI variant originated from ecliptic pHluorin

Depositor

Bradley Baker

Article

Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.

Insert

Photoactivatable_Bongwoori-R3

Use

Tags

Expression

Mammalian

Mutation

L64F, T65S, V163A, T203H, A227D

Promoter

CMV

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge