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Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPExpressionMutationPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
Plasmid#103865PurposeCatalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).DepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationContains the H133A and H1058A mutations which ina…PromoterEF1aAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64
Plasmid#180263PurposeLentiviral expression vector for dCas9-VP64 in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3G-LSD1-dCas9-P2A-mCherry
Plasmid#138462Purpose2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherryDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rad51AP1 B12.3 gRNA
Plasmid#90869Purpose3rd generation lentiviral gRNA plasmid targeting human Rad51AP1DepositorInsertRad51AP1 (Guide Designation B12.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC014 - LwCas13a-msfGFP
Plasmid#91902PurposeExpresses active LwCas13a for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPRTagsNLS and msfGFP-NLS-3xHAExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
sg14x(MS2) MUC4.1
Plasmid#101153PurposegRNA with 14 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRM1 E1.3 gRNA
Plasmid#90880Purpose3rd generation lentiviral gRNA plasmid targeting human RRM1DepositorInsertRRM1 (Guide Designation E1.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_zsGreen1
Plasmid#138463Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1DepositorInsertzsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
NLS-SpCas9mSA-His
Plasmid#182951PurposeExpress SpCas9mSA in bacteriaDepositorInsertNLS-SpCas9mSA-His
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX333-T2A-EGFP
Plasmid#197417PurposeSpCas9-T2A-EGFP with dual sgRNA cloning backbonesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBh; U6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-vpr
Plasmid#89985Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locusDepositorInsertdCas9-VPR
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK3079
Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only