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Plasmid#207019PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT74
Plasmid#218730PurposeExpresses N-terminally EGFP-tagged IFT74 in mammalian cellsDepositorInsertIFT74 (IFT74 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro SPNS1_sg2
Plasmid#218531PurposesgRNA targeting human SPNS1DepositorInsertSPNS1 (SPNS1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro SPNS1_sg1
Plasmid#218530PurposesgRNA targeting human SPNS1DepositorInsertSPNS1 (SPNS1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Nova1-GFP
Plasmid#217033PurposeOverexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neuronsDepositorInsertNova1 (Nova1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8-MET15
Plasmid#207055PurposeExpression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationPromoterpATG8Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆RPromoterpATG8Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆GR-MET15
Plasmid#207033PurposeExpression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆GRPromoterpATG8Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3-LN
Plasmid#207129PurposeLentiviral plasmid to express mouse Mettl3 Lambda peptide C-terDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HA and Lambda Peptide (LN)ExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Il13
Plasmid#207125PurposeLuciferase vector containing 3'UTR for mouse Il13DepositorInsertInterleukin 13 (Il13) 3'UTR (Il13 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Il13 5xBoxB
Plasmid#207128PurposeTethered luciferase reporter for mouse Il13 BoxBDepositorInsertInterleukin 13 (Il13) 3'UTR (Il13 Mouse)
UseLuciferaseTags5xBoxBExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 pCAGGS
Plasmid#206101Purposeexpression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expressionDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TSAA
Plasmid#206179Purposein vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194ADepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS192A, S194APromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TAAS
Plasmid#206180Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192ADepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS190A, S192APromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TASA
Plasmid#206181Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194ADepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS190A, S194APromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2mut
Plasmid#203606PurposeReporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequencesDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseTagsExpressionMammalianMutationmiR-27b target sequences in the 3'UTR were m…PromoterSV40Available sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSII-TK-B4GALT3
Plasmid#203598PurposeLentiviral vector to express B4GALT3 in mammalian cellsDepositorInsertbeta-1,4-galactosyltransferase 3 (B4GALT3 Human)
UseLentiviralTagsExpressionMammalianMutationWTPromoterTKAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSII-TK-MAIP1
Plasmid#203599PurposeLentiviral vector to express MAIP1 in mammalian cellsDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLentiviralTagsExpressionMammalianMutationWTPromoterTKAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-B4GALT3
Plasmid#203601PurposeReporter plasmid carrying B4GALT3 3'UTRDepositorInsertbeta-1,4-galactosyltransferase 3 (B4GALT3 Human)
UseLuciferaseTagsExpressionMammalianMutationnt 1800-2330 3'UTRPromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-MAIP1
Plasmid#203603PurposeReporter plasmid carrying MAIP1 3'UTRDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_B
Plasmid#206135PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_A
Plasmid#206134PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-P
Plasmid#203747PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
trLATm1PtoLmEos3.2
Plasmid#203749PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm1 (PtoL) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationP8L, P17LPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-Villin
Plasmid#200301PurposeBacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35DepositorInsertbeta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-lambdaN-HA-DmNot1_1497-1644_W
Plasmid#147872PurposeInsect Expression of DmNot1_1497-1644DepositorInsertDmNot1_1497-1644 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1387-1699_W
Plasmid#147847PurposeInsect Expression of DmNot1_1387-1699DepositorInsertDmNot1_1387-1699 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1387-1717_W
Plasmid#147848PurposeInsect Expression of DmNot1_1387-1717DepositorInsertDmNot1_1387-1717 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1467-1699_W
Plasmid#147849PurposeInsect Expression of DmNot1_1467-1699DepositorInsertDmNot1_1467-1699 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1467-1717_W
Plasmid#147850PurposeInsect Expression of DmNot1_1467-1717DepositorInsertDmNot1_1467-1717 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1497-1644_W
Plasmid#147857PurposeInsect Expression of DmNot1_1497-1644DepositorInsertDmNot1_1497-1644 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182_1-634_W
Plasmid#147830PurposeInsect Expression of DmGW182_1-634DepositorInsertDmGW182_1-634 (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-CTAP-PrP-SBP-DmCaf40-N63AF65AC69YV76Y_W
Plasmid#147833PurposeInsect Expression of DmCaf40-N63AF65AC69YV76YDepositorInsertDmCaf40-N63AF65AC69YV76Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-CTAP-PrP-SBP-DmCaf40-NFCVYAtoAAYYAF_W
Plasmid#147834PurposeInsect Expression of DmCaf40-NFCVYAtoAAYYAFDepositorInsertDmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-Q294AN296F-dsRNAres_W
Plasmid#147840PurposeInsect Expression of DmMe31B-Q294AAN296F-dsRNAresDepositorInsertDmMe31B-Q294AAN296F-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-Q294AN296FR347A-dsRNAres_W
Plasmid#147841PurposeInsect Expression of DmMe31B-Q294AAN296FR347A-dsRNAresDepositorInsertDmMe31B-Q294AAN296FR347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-R347A-dsRNAres_W
Plasmid#147842PurposeInsect Expression of DmMe31B-R347A-dsRNAresDepositorInsertDmMe31B-R347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-S291A-dsRNAres_W
Plasmid#147843PurposeInsect Expression of DmMe31B-S291A-dsRNAresDepositorInsertDmMe31B-S291A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF1_33-52-GB1_AE
Plasmid#148552PurposeBacterial Expression of HsGIGYF1_33-52DepositorInsertHsGIGYF1_33-52 (GIGYF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF1_33-71_AE
Plasmid#148553PurposeBacterial Expression of HsGIGYF1_33-71DepositorInsertHsGIGYF1_33-71 (GIGYF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF1_33-71-GB1_AE
Plasmid#148554PurposeBacterial Expression of HsGIGYF1_33-71DepositorInsertHsGIGYF1_33-71 (GIGYF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A_AE
Plasmid#148555PurposeBacterial Expression of HsGIGYF1_33-103-E44AE45FQ87ADepositorInsertHsGIGYF1_33-103-E44AE45FQ87A (GIGYF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF1_33-103-GB1_AE
Plasmid#148557PurposeBacterial Expression of HsGIGYF1_33-103DepositorInsertHsGIGYF1_33-103 (GIGYF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-QHTKtoAAAA-dsRNAres_AC
Plasmid#148391PurposeInsect Expression of DmMe31B-QHTKtoAAAA-dsRNAresDepositorInsertDmMe31B-QHTKtoAAAA-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-RFQNRtoEDAFA-dsRNAres_AC
Plasmid#148393PurposeInsect Expression of DmMe31B-RFQNRtoEDAFA-dsRNAresDepositorInsertDmMe31B-RFQNRtoEDAFA-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1149-1962-dsRNAres_AC
Plasmid#148399PurposeInsect Expression of DmNot1_1149-1962-dsRNAresDepositorInsertDmNot1_1149-1962-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only