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Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMDC32B-AtTAS1c-D2-B/c
Plasmid#137884PurposePlant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseTagsExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …Promoter2x35SAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-NbVHH05-mCherry
Plasmid#171572PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cellsDepositorInsertNbVHH05-mCherry (UBE2J1 Human)
UseTagsmCherryExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-JIP4
Plasmid#164621PurposeExpresses JIP4 in mammalian cells with a mNeonGreen tagDepositorInsertJIP4 (SPAG9 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-CIBN
Plasmid#102251PurposeExpresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-VCPIP1-Strep-HA
Plasmid#113482PurposeExpression of human VCPIP1 with C-terminal strep-HA tagDepositorInsertVCPIP1 (VCPIP1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP1.GFP
Plasmid#109139PurposeGFP-tagged CYFIP1 expression constructDepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP16 (B1014)
Plasmid#92036PurposeExpresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.DepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 (intact NLS) with VP16 activ…ExpressionMammalianMutationPromoterT7Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorUseTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-JIP4
Plasmid#164633PurposeExpresses JIP4 in mammalian cells with a 2xmyc tagDepositorInsertJIP4 (SPAG9 Human)
UseTags2xmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI4-3×FLAG
Plasmid#215512PurposeExpresses FLAG tagged human WIPI4.DepositorInsertWD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)
UseRetroviralTags3×FLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAW-NbVHH05-GFP
Plasmid#171570PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cellsDepositorInsertNbVHH05-GFP (UBE2J1 Human)
UseTagsGFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-ATRX ADD
Plasmid#59698PurposeContains histone modification interacting domain (ATRX ADD)DepositorInsertATRX ADD (ATRX Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-KDM4A double Tudor
Plasmid#59699PurposeContains histone modification interacting domain (KDM4A double Tudor)DepositorInsertKDM4A double Tudor (KDM4A Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only