Addgene

Showing: 2741 - 2760 of 150461 results

Results per page:

Sort results by:

pcDNA3-RPS6 (pDD1902)

Plasmid

#52913

Purpose

contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

Depositor

Dirk Dittmer

Article

Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

Insert

HAS6 (RPS6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
N174-MCS (Puro)

Plasmid

#81068

Purpose

Lentiviral vector for constitutive gene expression.

Depositor

Adam Karpf

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.2-ccdB-3xFLAG-V5

Plasmid

#87071

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NLS-RfxCas13d-mcherry-NLS-Flag

Plasmid

#165077

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-DTAG-CTERM-GFP

Plasmid

#185765

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Expression

Mutation

none

Promoter

SFFV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-WHaloCaMP1a-EGFP

Plasmid

#205303

Purpose

Expression of WHaloCaMP1a-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1230

Plasmid

#144092

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

AFF3 (AFF3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-PPARA

Plasmid

#169019

Purpose

Expression of PPARA driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human ppara (PPARA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKNOCK-Cm

Plasmid

#46261

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX puro TRIM21-GFP

Plasmid

#116941

Purpose

Expression of TRIM21-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 - GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_psychLight2

Plasmid

#163910

Purpose

Expresses psychLight2 in mammalian cells

Depositor

Lin Tian

Article

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight2

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CD40L-SrtA

Plasmid

#121167

Purpose

pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

CD40L-SrtA

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (for expressing sgRNA)

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_047

Plasmid

#107145

Purpose

measures Cas9 activity

Depositor

David Root

Insert

GFP + sgRNA for GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet_Giantin_C1

Plasmid

#85048

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE 80L Kan T7WT

Plasmid

#102790

Purpose

Expresses T7 RNA polymerase

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

T7 RNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AAVS1-MAFB-IRF8-CEBPA

Plasmid

#190720

Purpose

For microglia differentiation from iPSC

Depositor

Li Gan

Article

Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

Insert

MAFB-IRF8-CEBPA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN251

Plasmid

#188471

Purpose

Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

enCas12a-HF (gift from Dr. Keith Joung)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge