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Plasmid#115909Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP (ACTB Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3543
Plasmid#145019PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCSDC2 (CSDC2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsNoneExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN- myc ULK1 wt
Plasmid#27626DepositorInsertmouse myc ULK1 (Myc Mouse)
UseRetroviralTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A
Plasmid#74605Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-Actin-C-18
Plasmid#54863PurposeLocalization: Actin, Excitation: 568, Emission: 592DepositorInsertActin (ACTB Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cntn1-AP-His
Plasmid#71939PurposeExpresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertCntn1 (Cntn1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARΔ142–337
Plasmid#89106PurposeMammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1DepositorInserthAR delta 142-337 (AR Human)
UseTagsExpressionMammalianMutationhAR delta 142-337PromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-LAP-KNL1-M3
Plasmid#115896PurposeUsed for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertKNL1-M3 (KNL1 Human)
UseTagsEYFPExpressionMammalianMutationFragment of KNL1 containing three KNL1 repeat seq…PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A
Plasmid#74606Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103A, W120APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 C-terminal half
Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0959
Plasmid#141766PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertERF (ERF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant
Plasmid#72616PurposeExpresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Actin-C-18
Plasmid#57571PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant
Plasmid#72922PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationPromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-EGFP-5'3'short
Plasmid#129750PurposeGene targeting in marmoset cellsDepositorInsertACTB (ACTB C. jacchus (common marmost))
UseMarmoset targetingTagsEGFPExpressionMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 - myc ULK1 k46I
Plasmid#27624DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseGateway donr vectorTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
AT1G64210 I9_pECIA2
Plasmid#114984PurposeBait vector AT1G64210 I9_pECIA2 should be used with prey vector AT1G64210 I9_pECIA14.DepositorInsertAT1G64210 (AT1G64210 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only