Addgene

Showing: 2721 - 2740 of 6704 results

Results per page:

Sort results by:

pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pONSY-mCherry

Plasmid

#111874

Purpose

This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Expression

Mutation

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCin4-3xFLAG-MKL1 S449A/T450A/S454A

Plasmid

#19845

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-Arkadia-gRNA1

Plasmid

#180374

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-NE-EGFP-IRES-Pac

Plasmid

#183617

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

Insert

EGFP

Use

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-syn-fRGECO2

Plasmid

#125244

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

Expression

Mammalian

Mutation

W44Y, D432A

Promoter

synapsin

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4 mIL-17 1.1kb promoter

Plasmid

#20125

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pCFJ907

Plasmid

#44473

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKsubDD-YPet-NES

Plasmid

#84633

Purpose

Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

JNKsubDD-YPet-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 13: 5’ATF4.uORF2AUA.GFP

Plasmid

#21860

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF2 was converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
BDS-2 3x MUT (p53 binding site)

Plasmid

#16516

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant p53 binding sites

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgSLC19A1

Plasmid

#102314

Purpose

genetic depletion of SLC19A1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgSLC19A1 (SLC19A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-BRDCH2 (1079-1317)

Plasmid

#99339

Purpose

Bacterial expression of mouse CBP BRDCH2 domains as a GST fusion

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

mouse CBP BRDCH2 (1079-1317) (Crebbp Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 12: 5’ATF4.uORF1AUA.GFP

Plasmid

#21859

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF1 was converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK5_009

Plasmid

#123853

Purpose

Encodes ConR6 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK5_010

Plasmid

#123854

Purpose

Encodes ConR7 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge