-
Plasmid#118800PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationAll FYW to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct10
Plasmid#28203DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa1-105 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-T2A-miniSOG-C-Far
Plasmid#193915PurposeExpresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV productionDepositorInsertminiSOG
UseAAVTagsFarnesylation signal sequence and Flag tagExpressionMammalianMutationPromoterhSynAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGASΔN-HA (murine)
Plasmid#130915PurposeExpresses murine cGASΔN (aa148-507)-HA; Puromycin selection markerDepositorInsertcGASΔN murine
UseTagsHAExpressionMammalianMutationmurine cGAS catalytic domain (aa148-507)PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1415 GFP-TDP43 VLIM-S
Plasmid#133323PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationALL VLIM in the TDP43 IDRs mutated to SPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct9
Plasmid#28202DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa1-193 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-44
Plasmid#91780PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP3-44
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV epArcLight
Plasmid#85803Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertepArcLight
UseTagsExpressionMammalianMutationCi-VSP contains R217Q mutation; ecliptic pHluorin…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(Q258K)
Plasmid#133077PurposeX. laevis expression vector. It will generate the human TRAAK channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
UseTagsExpressionMutationQ258KPromoterT7Available sinceOct. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS-N-HA (murine)
Plasmid#130916PurposeExpresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection markerDepositorInsertmurine cGAS N terminus
UseTagsHAExpressionMammalianMutationmurine cGAS N terminus (aa 1-147)PromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1412 GFP-TDP43 FYW-L
Plasmid#118801PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationAll FYW to L in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-AGM-TAN1 vpr
Plasmid#115819PurposeEncodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance proteinDepositorInsertSIVAGM Vpr (TAN1)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO GFR alpha 1
Plasmid#119898PurposeMouse GFR alpha 1 In situ hybridization probeDepositorInsertGDNF family receptor alpha-1 precursor (Gfra1 Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE human IRS-1 CAAX
Plasmid#11237DepositorInsertIRS-1 (IRS1 Human)
UseRetroviralTagsCAAXExpressionMammalianMutationC-terminal prenylation motif makes IRS-1 localize…PromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5Flag2-P2A-smAID-mClover
Plasmid#110657PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5Flag2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-WW1-mutant
Plasmid#17795DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 to Alanine; Proline 202 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-WW2-mutant
Plasmid#17796DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 258 to Alanine Proline 261 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
Myc-PCDHGC5
Plasmid#119935PurposeProtocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
UseTags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5HA2-bglpA
Plasmid#110655PurposeLentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5HA2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:hTRAAK(G124I)
Plasmid#130672PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFPDepositorInsertKCNK4 (KCNK4 Human)
UseTagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationN104Q, N108Q, G124IPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gucy1b2-D-IRES-tauGFP-pA-ACNF TV
Plasmid#105208Purposetargeting vector to generate a Gucy1b2-D-IRES-tauGFP mouse strain.DepositorInsertGucy1b2-D-IRES-tauGFP-pA-ACNF (Gucy1b2 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNF149 shRNA-TRE
Plasmid#225337PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertRNF149 shRNA (Rnf149 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.4
Plasmid#200230PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV1.4
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaVB2
Plasmid#200231PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV beta 2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.4-N830T
Plasmid#200285PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV1.4
UseTagsExpressionMammalianMutationN830TPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.5
Plasmid#200232PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis Nav1.5
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.4-N432T
Plasmid#200284PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV1.4
UseTagsExpressionMammalianMutationN432TPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.4-N11306T
Plasmid#200286PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV1.4
UseTagsExpressionMammalianMutationN11306TPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.4-N1609T
Plasmid#200287PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV1.4
UseTagsExpressionMammalianMutationN1609TPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pum NaV1.4-N1609A
Plasmid#200288PurposeExpression in mammalian cells or oocytesDepositorInsertPitohui uropygialis meridionalis NaV1.4
UseTagsExpressionMammalianMutationN1609APromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase shRNA-TRE
Plasmid#225339PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertLuciferase shRNA (LOC116160065 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF130 shRNA-TRE
Plasmid#225336PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertRNF130 shRNA (Rnf130 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3XFLAG-NESm-NFATC2IP
Plasmid#221445PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with mutant nuclear export signal (NESm) at N-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlag and Mutant Nuclear Export Signal (mNES)ExpressionMammalianMutationmutant NES (NESm: NLVDLQKKLEEAKADEQQ (PMID: 10739…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP D394R
Plasmid#221211PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interactionDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationD394R (charge-reverting mutation for interaction …PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-eGFP-NFATC2IP D394R
Plasmid#221436PurposeMammalian expression vector of eGFP tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interactionDepositorInsertNFATC2IP (NFATC2IP Human)
UseTagseGFPExpressionMammalianMutationD394R (charge-reverting mutation for interaction …PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP ∆SLD1
Plasmid#221209PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with SUMO-like domain 1 (SLD1, aa 256-344) deletedDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationSUMO-like domain 1 (SLD1, aa 256-344) deleted, 1s…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP ∆SLD2
Plasmid#221210PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with SUMO-like domain 2 (SLD2, aa 345-419) deletedDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationSUMO-like domain 2 (SLD2, aa 345-419) deleted, 1s…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
plenti hSyn AldoA
Plasmid#220916PurposeNeuronal specific expression of AldoADepositorInsertAldoA (ALDOA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PGK1-HALO
Plasmid#220910PurposeNeuronal specific expression of the fusion construct PGK1-HALODepositorInsertPGK1-HALO (PGK1 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI2B-3×FLAG
Plasmid#215510PurposeExpresses FLAG tagged human WIPI2B.DepositorInsertWD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
UseRetroviralTags3×FLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4169
Plasmid#200108PurposePrig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFPDepositorInsertsnb-1 (snb-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4203
Plasmid#200160PurposePrig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4018
Plasmid#200043PurposePrig-3 FRT let858 (stop) FRT zif-1 SL2 EBFP unc-54 3' UTR C.elegans AVA and other neurons expression of zif EBFPDepositorInsertzif-1 (zif-1 Nematode)
UseTagsEBFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00028 (aka pTS0057)
Plasmid#200832PurposeAAVS1 guide RNA cloned into the lentiSAM puro backboneDepositorInsertAAVS1 guide RNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-RPT6-FLAG
Plasmid#205416PurposeExpresses dCas9-RPT6 with FLAG labelDepositorInsertdCas9-RPT6 (Psmc5 Rat)
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSynapsin-Nluc-EPAC-VV
Plasmid#204846PurposeProduce lentivirus encoding Nluc-EPAC sensor in neuronal cell typeDepositorInsertNluc-EPAC sensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mRuby3-2A-dCre
Plasmid#203845PurposeFlp-dependent enzymatically inactive Cre and mRuby3 expressionDepositorInsertdCre
UseAAVTagsmRuby3-2AExpressionMutationEnzymatically inactive CrePromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only