Addgene

148,416 results

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBS hTSP-4

Plasmid

#13001

Depositor

Jack Lawler

Article

Lawler et al J Biol Chem. 1995 Feb 10. 270(6):2809-14

Insert

TSP-4 (THBS4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pNCS mCherry

Plasmid

#91769

Purpose

Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Cherry

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

See Depositor Comments

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo Flag HAUSP

Plasmid

#16655

Depositor

Bert Vogelstein

Article

Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

Insert

HAUSP (USP7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 WT

Plasmid

#8317

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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eforRed chromoprotein

Plasmid

#117837

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, eforRed

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1

Plasmid

#186829

Purpose

Expresses FLAG-BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha-pcDNA3.EGFP

Plasmid

#13009

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pDest-Sumo

Plasmid

#106980

Purpose

LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

Depositor

Han Choe

Article

Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

Type

Empty backbone

Use

Tags

His8-Sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

Plasmid

#69100

Purpose

URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

Depositor

David Botstein

Inserts

yZ3EV cassette
Z3 promoter
yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12V

Plasmid

#81665

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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Cerulean-tagged Apollo-NADP+

Plasmid

#71800

Purpose

Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSFG-GFP

Plasmid

#22199

Depositor

Ananda Roy

Article

Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

Insert

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pWXC077

Plasmid

#222960

Purpose

C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Article

Cao et al bioRxiv 2024.06.11.598534

Insert

mScarlet3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-YY1

Plasmid

#154943

Purpose

Knocking down of human YY1 through shRNA and amiRNA targeting YY1

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

shRNA and amiRNA against YY1 (YY1 Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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cyto-iATPSnFR1.0

Plasmid

#102550

Purpose

cytoplasmic ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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