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Plasmid#92289PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cellsDepositorInsertdGW182 CED 7W7A (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215 with the following point …PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-59
Plasmid#92261PurposePlasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorInsertdGW182 (gw Fly)
UseTagsHAExpressionInsectMutationaa 940-1215PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-1
Plasmid#86387PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-3
Plasmid#86389PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-4
Plasmid#86390PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3_zfh1_CP-candidate_luc+
Plasmid#86391PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activityDepositorInsertD. melanogaster zfh1 enhancer
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ssp3
Plasmid#86382PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ssp3 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ham
Plasmid#86383PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ham enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-sgl
Plasmid#86384PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster sgl enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ncm
Plasmid#86385PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ncm enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1_spikeIn-2
Plasmid#86388PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMOdeltaC
Plasmid#85697Purposeexpression of GFP-SUMO1 delta C in Drosophila cellsDepositorInsertSUMO (Sumo Fly)
UseTagsGFPExpressionInsectMutationnon-conjugatable SUMO, lacking the C-terminal gly…PromoterpAc5Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS-Xbp1spliced
Plasmid#80628Purposeexpression of Xbp1 RB isoform in DrosophilaDepositorInsertXbp1 (Xbp1 Fly)
UseTagsExpressionInsectMutationRB isoformPromoterhsp70Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 AN+
Plasmid#78240PurposeOne of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).DepositorInsertsloxP
sequence "A" with NheI site
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 BS-
Plasmid#78239PurposeOne of four plasmids needed to create the double Holliday junction substrate (DHJS).DepositorInsertsloxP
sequence "A"
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 AS+
Plasmid#78236PurposeOne of four plasmids needed to create the double Holliday junction substrate (DHJS).DepositorInsertsloxP
sequence "A"
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-dSestrin (CG11299-PD)
Plasmid#73675PurposeoverexpressionDepositorInsertSesn (variant D) (Sesn Fly)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1E07
Plasmid#71086PurposeGateway entry cloneDepositorInsertTaf13 (Taf13 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C01
Plasmid#70944PurposeGateway entry cloneDepositorInsertOrc1 (Orc1 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only