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Plasmid#79409PurposeExpresses C-terminally HA-tagged Arf1(WT) in mammalian cellsDepositorInsertArf1 (ARF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2(535, L348F)-mCherry
Plasmid#75372PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherryDepositorInsertCRY2 residues 1-535 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationL348FPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon hPGK
Plasmid#206230PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
PPRE-pNL1.3
Plasmid#84394PurposeSecreted Luciferase reporter for PPARgamma activityDepositorInsertPPARG response element (PPARG Human)
UseTagssecNlucExpressionMammalianMutationPromoterPPREAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1803
Plasmid#143318PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertUSF1 (USF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40
Plasmid#194690PurposehSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_06-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIB81-pmGFP
Plasmid#75366PurposeExpresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on endDepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsprenylated EGFPExpressionMammalianMutationresidues 1-81 of CIB1PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40
Plasmid#194691PurposehSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_on-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR(W349R)-mCherry
Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEO-CaMKII-TVA-P2A-N2cG
Plasmid#172363PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinaseDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.tRFP
Plasmid#169941PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-mCherry
Plasmid#135189PurposeExpresses TRPML1 with C-terminus mCherry tag in mammalian cells.DepositorInsertTRPML1 (Mcoln1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPING
Plasmid#26046DepositorInsertSS peptide-LacZ-KHHHHHH
UseTagsKHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-LACTB-dAPEX2
Plasmid#117180PurposePlasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2F
Plasmid#15800DepositorInsertUbiquitin conjugating enzyme UbE2F (UBE2F Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Flag-Ube2v2
Plasmid#110070PurposeExpress Halo-Tev-Flag-Ube2v2 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v2 (UBE2V2 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only