Skip to main content
Addgene
Showing: 2701 - 2720 of 16189 results

  3. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid
    #115891
    Purpose
    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038
    Insert
    Archon1-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115891-plasmid-map-sequence-id-242072

    Enlarge

  4. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

    Enlarge

  5. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

    Enlarge

  6. L5-LexAp65-L2

    Plasmid
    #41437
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    41437-plasmid-map-sequence-id-55247

    Enlarge

  7. L1-10XQUAS-L4

    Plasmid
    #41436
    Depositor
    Steve Stowers
    Insert
    10XQUAS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41436-plasmid-map-sequence-id-55246

    Enlarge

  8. L1-10XQUAS-R5

    Plasmid
    #41435
    Depositor
    Steve Stowers
    Insert
    10XQUAS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41435-plasmid-map-sequence-id-55245

    Enlarge

  9. pENTR L1-13XLexAop2-L4

    Plasmid
    #41434
    Depositor
    Steve Stowers
    Insert
    13XLexAop2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41434-plasmid-map-sequence-id-55244

    Enlarge

  10. R4-LexAp65-R3

    Plasmid
    #41438
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    41438-plasmid-map-sequence-id-55248

    Enlarge

  11. pENTR L1-13XLexAop2-R5

    Plasmid
    #41433
    Depositor
    Steve Stowers
    Insert
    13XLexAop2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41433-plasmid-map-sequence-id-55242

    Enlarge

  13. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

    Enlarge

  14. pQhsCDtRNA-

    Plasmid
    #86015
    Purpose
    Multi-gene expression system with human box C/D proteins with U14
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86015-plasmid-map-sequence-id-169486

    Enlarge

  15. pUltra-Plus

    Plasmid
    #161775
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
    Depositor
    Jorge Luis Arias-Arias
    Type
    Empty backbone
    Use
    Lentiviral; Multicistronic
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161775-plasmid-map-sequence-id-316822

    Enlarge

  16. pUAST-3x attB

    Plasmid
    #220329
    Purpose
    includes 3 standard UAS cassettes
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220329-plasmid-map-sequence-id-442491

    Enlarge

  17. 2xUAS-1xhsp70-attB

    Plasmid
    #220317
    Purpose
    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220317-plasmid-map-sequence-id-442487

    Enlarge

  18. pJK531

    Plasmid
    #72512
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72512-plasmid-map-sequence-id-132538

    Enlarge

  19. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

    Enlarge

  20. pJK539

    Plasmid
    #72515
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72515-plasmid-map-sequence-id-132543

    Enlarge

Showing: 2701 - 2720 of 16189 results