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Plasmid#155103Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_BFP2
Plasmid#155097PurposeLentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_LacZ_sgRNA
Plasmid#155108Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBOB-N-3HA-GK_v1
Plasmid#134275PurposeLentivector encoding 3XHA-tagged GK (isoform 1)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation524 amino acids, isoform 1PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS10-riboE-dddAI
Plasmid#186561PurposeRegulated expression of the DddA immunity determinant (DddAI) in FirmicutesDepositorInsertdddAI
UseTagsExpressionBacterialMutationPromoterpSpac(hy)Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)
Plasmid#171099PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.DepositorInserthSpCas9 (Ascl1 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_Luciferase_sgRNA
Plasmid#155109Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LuciferaseDepositorInsertLuciferase_sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_LacZ_sgRNA_2
Plasmid#155093Purposelentiviral plasmid expressing Cas9 and gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_Luciferase_sgRNA
Plasmid#155094Purposelentiviral plasmid expressing Cas9 and gRNA targeting LuciferaseDepositorInsertLuciferase_sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-3HA-GK_v2
Plasmid#134277PurposeLentivector encoding 3XHA-tagged GK (isoform 2)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation553 amino acids, isoform 2PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK_v1
Plasmid#134270PurposeLentivector encoding Flag-tagged GK (isoform 1)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk isoform 1; 524 amino acidsPromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D443N)
Plasmid#134293PurposeExpresses GK5 (isoform 2)-D443N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acid 443 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D280N)
Plasmid#134290PurposeExpresses GK5 (isoform 2)-D280N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acid 280 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N)
Plasmid#134289PurposeExpresses GK5 (isoform 2)-D30N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acid 30 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-Flag-GK5_v2
Plasmid#134282PurposeExpresses Flag-tagged GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-Flag-GK5_v1
Plasmid#134281PurposeExpresses Flag-tagged GK5 (isoform 1) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-GK5_v2
Plasmid#134280PurposeExpresses GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-GK5_v1
Plasmid#134279PurposeExpresses GK5 (isoform 1) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only