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Plasmid#197328PurposePlasmid expressing an antigen targeting NECL4/SynCAM4DepositorInsertSynCAM4 (Cadm4 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH341-SUP45(139-437)-GalBD
Plasmid#29741DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 139-437 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH339-SUP45(1-138)-GalBD
Plasmid#29739DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 1-138 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH342-SUP45(276-437)-GalBD
Plasmid#29742DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 276-437 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycExpressionMutationPromoterHuC(elavl3)Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-AR
Plasmid#213819PurposeLenti-viral expression of human androgen receptor wild-type (WT).DepositorInsertAR (human androgen receptor) (AR Human)
UseLentiviralTagsFlag (N terminal on backbone)ExpressionMammalianMutationNo, it is AR wild-type.PromoterEF-1aAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-C619Y
Plasmid#171222PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.DepositorInsertAR-C619Y (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C619YPromoterEF-1aAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-moxGFP-P2A-T2A-Puro
Plasmid#176893PurposeSleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interestDepositorInsertmoxGFP-P2A-T2A
UseTransposonTagsmoxGFPExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-TAZ S89A
Plasmid#179910PurposeConstruct for expressing an activated form of TAZ (encoding be gene WWTR1)DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseTags3X Flag-tagExpressionMammalianMutationSerine 89 mutated to AlaninePromoterEF1aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-Lb-Cas12a-2xNLS
Plasmid#155046PurposeLentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoterDepositorInsertLbCas12a
UseCRISPR and LentiviralTagsNucleoplasmin NLS, Myc-tag and SV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SIRT7_H187Y
Plasmid#53151Purposeexpression vector for catalytically inactive human Sirtuin7DepositorInsertSirt7 (SIRT7 Human)
UseTagsFLAGExpressionMammalianMutationH187Y, catalytic inactivePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Ezh2 Y637S-IRES-GFP
Plasmid#91887PurposeRetroviral expression of mouse Ezh2 with Y637SDepositorInsertEzh2 (Ezh2 Mouse)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationY637SPromoterMMLV gagAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-As-Cas12a-2xNLS
Plasmid#155047PurposeLentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoterDepositorInsertAsCas12a
UseCRISPR and LentiviralTagsNucleoplasmin NLS, Myc-tag and SV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Ezh2 delta SETdomain-IRES-GFP
Plasmid#91885PurposeRetroviral expression of mouse Ezh2 with a SET domain deletionDepositorInsertEzh2 (Ezh2 Mouse)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationSET domain deletedPromoterMMLV gagAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLXSN p200 CUX1
Plasmid#90470PurposeRetroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectivelyDepositorInsertCUX1 (CUX1 Human)
UseRetroviralTagsHa and mycExpressionMammalianMutationPromoterMoloney murine leukemia virus long terminal repeatAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSCRET2(#323)
Plasmid#184058Purposecyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesisDepositorInsert6myc-CRE-ERT2
UseSynthetic BiologyTags6xMycExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-mBVRA
Plasmid#100286PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for mouse BVRA
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only