LV-AR-C619Y
(Plasmid
#171222)
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PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171222 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLenti-viral-Hygro
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAR-C619Y
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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MutationAR-C619Y
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter EF-1a
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The mutation occurs at the end of androgen receptor zinc finger 2 and this AR mutant is defective in transactivation but not nuclear import.
Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LV-AR-C619Y was a gift from Steven Balk (Addgene plasmid # 171222 ; http://n2t.net/addgene:171222 ; RRID:Addgene_171222) -
For your References section:
Androgen receptor and MYC equilibration centralizes on developmental super-enhancer. Guo H, Wu Y, Nouri M, Spisak S, Russo JW, Sowalsky AG, Pomerantz MM, Wei Z, Korthauer K, Seo JH, Wang L, Arai S, Freedman ML, He HH, Chen S, Balk SP. Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y. 10.1038/s41467-021-27077-y PubMed 34911936