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Showing: 2701 - 2720 of 8409 results

  2. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  4. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only

  5. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  7. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169389-plasmid-map-sequence-id-336165

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  8. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

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  9. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

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  11. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155251-plasmid-map-sequence-id-313953

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  12. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

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  13. pmCellFree_KA3_SARS-COV-2_RBD

    Plasmid
    #169405
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 RBD domain of S protein with C-term mCherry
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _RBD (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169405-plasmid-map-sequence-id-336878

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  14. pmCellFree_KA1_SARS-COV-2_ORF10

    Plasmid
    #169398
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169398-plasmid-map-sequence-id-336872

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  15. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169402-plasmid-map-sequence-id-336167

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  16. pmCellFree_KA1_SARS-COV-2_M

    Plasmid
    #169400
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus M with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _M (M SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169400-plasmid-map-sequence-id-336863

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  17. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 2 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68350-plasmid-map-sequence-id-119622

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  18. pmCellFree_KA1_SARS-COV-2_E

    Plasmid
    #169399
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _E (E SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169399-plasmid-map-sequence-id-335422

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  19. pmCellFree_KA1_SARS-COV-2_ORF8

    Plasmid
    #169395
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169395-plasmid-map-sequence-id-336176

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  20. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

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Showing: 2701 - 2720 of 8409 results