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Plasmid#112967PurposeP element vector for transposon insertion of nondegradable Cyclin B into Drosophila genomeDepositorInsertcycB (CycB Fly)
UseP insertion vectorTagsExpressionMutationN-term deletion of first 46 amino acids, includes…PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C
Plasmid#113008PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila TralDepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 356-652PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Yes-10kb
Plasmid#112996PurposeP element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genomeDepositorInsert10Kb DAFC-62D amplicon
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAL4DBD-SuUR f.l
Plasmid#112995PurposeP element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genomeDepositorInsertSuUR (SuUR Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C 15A
Plasmid#113007PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)DepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationPNG phosphorylation sites mutated to APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
E2F1 in pGEX4t
Plasmid#112976PurposeFor protein expression and purification of Drosophila E2F1DepositorInsertE2F1 (E2f1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
mr-GST N-term fusion
Plasmid#112983PurposeFor protein expression and purification of N-terminus of Drosophila mr (APC2)DepositorInsertmr (APC2) (mr Fly)
UseTagsExpressionBacterialMutationN-term onlyPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG172-STII PLU-HIS
Plasmid#112992PurposeBaculovirus expression of Drosophila png172-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationpng172 mutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-STII PLU-HIS
Plasmid#112993PurposeBaculovirus expression of Drosophila png-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15D
Plasmid#113010PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutationsDepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutation15 PNG phosphorylation sites mutated to DPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mr-GST full length
Plasmid#112982PurposeFor protein expression and purification of full-length Drosophila mr (APC2)DepositorInsertmr (APC2) (mr Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-Flag-dTSC2 S1338A
Plasmid#111808PurposeInsect Expression of dTSC2DepositorInsertdTSC2 (TSC2 Fly)
UseTags3xFlagExpressionInsectMutationS1338APromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati Exons 1-3
Plasmid#110113PurposeDrosophila dati minigene plasmid that expresses both a linear RNA and a circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 dATG1 (1188)
Plasmid#66918Purposebacterial expression of dATG1DepositorInsertATG1
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMCh-76
Plasmid#92288PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorInsertdGW182 CED (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only