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Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
UseTags6xHis tag and NLSExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6-150
Plasmid#91777PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP6-150
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-3xFLAG-hphMX4
Plasmid#20755DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 GFP WT
Plasmid#35411DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
UseTagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Anti-GFP [N86/38.1R]
Plasmid#114492PurposeMammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.DepositorInsertanti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAX2
Plasmid#117398Purposeallelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switchDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hG9a
Plasmid#33024DepositorInserthG9a
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee
Plasmid#140836Purposemammalian expression of SpiCee, a buffer of calcium that prevents downstream signalingDepositorInsertSpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b
Plasmid#111621PurposeExpression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cellsDepositorInsertExonuclease 1b (EXO1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iC2
Plasmid#27345DepositorInsertSFFV promoter, IRES-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
UseTagsHisExpressionBacterialMutationPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only