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pENTR puroR R4-R3

Plasmid

#43965

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2013

Availability

Academic Institutions and Nonprofits only

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LZRS-GADD45A

Plasmid

#24954

Depositor

Paul Khavari

Article

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

GADD45A (GADD45A Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
p667-UBC-DYRK2-FullLength-V5-miniTurbo_IDG-K

Plasmid

#198010

Purpose

Gateway destination clone of DYRK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

DYRK2-V5-miniTurbo (DYRK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF2-CDS

Plasmid

#136040

Purpose

UPF2 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCGTTATGTTTGGTGGAAGAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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px459 EQR

Plasmid

#101732

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Use

Tags

Expression

Mammalian

Mutation

VQR

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

Plasmid

#61441

Purpose

Dox-inducible MyoD with mRNA tag for smFISH

Depositor

Charles Gersbach

Article

Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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p667-UBC-BCKDK-V5-miniTurbo_IDG-K

Plasmid

#158189

Purpose

Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

BCKDK-V5-miniTurbo (BCKDK Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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p7270 MSCV-N-HA-IRES-PURO Empty

Plasmid

#163302

Purpose

Empty vector control

Depositor

Peter Howley, Elizabeth White

Article

White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK16_IDG-K

Plasmid

#158173

Purpose

Gateway destination clone of CDK16 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK16 (CDK16 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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library backbone 3'LTR gRNA and barcode

Plasmid

#127397

Purpose

Digestion by BstXI and BamHI to release the backbone

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Type

Empty backbone

Use

Lentiviral

Tags

Puro-mCherry

Expression

Mutation

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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hTRAF2T117ApLPCX-HA-Flag/P875

Plasmid

#20230

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

T117A (a349g in TRAF2 CDS)

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

Plasmid

#163083

Purpose

Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

TagRFP-T

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2 long-wt

Plasmid

#21646

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2-short

Plasmid

#21655

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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PLKO.1-STAU1-3'UTR

Plasmid

#136049

Purpose

STAU1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CCATCACCACTGCTTTCTCTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

STAU1 (STAU1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP Raptor

Plasmid

#12587

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

Raptor (RPTOR Human)

Use

Retroviral

Tags

TAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101108

Purpose

Encodes dCas9(N) fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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