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Plasmid#210808PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAExpressionMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)
Plasmid#200977PurposeMammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)DepositorInsertEFNB2 (EFNB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationD62Q-Q130L-V167L (increase specificity towards he…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q)
Plasmid#200979PurposeMammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
UseTagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q mutation fo…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)
Plasmid#200980PurposeMammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
UseTagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q-Q130L-V167L…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (D62Q)
Plasmid#200976PurposeMammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)DepositorInsertEFNB2 (EFNB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationD62Q (increases specificity towards henipaviral G…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_ER
Plasmid#182818PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_ER
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_PM
Plasmid#182819PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_PM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_cyto
Plasmid#182820PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_cyto
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_ER
Plasmid#182821PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_ER
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_PM
Plasmid#182822PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_PM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_cyto
Plasmid#182823PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_cyto
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1-mCherry-Puro
Plasmid#193766PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBGE2.0
Plasmid#165987PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
tetracycline resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-mADAR2-short
Plasmid#175588Purposemammalian expression of mouse short Adar2, blasticidine selectionDepositorInsertShort Mouse Adar2
UseTagsFlagExpressionMammalianMutationPromotercmvAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-DIO-GCaMP6s
Plasmid#184284PurposeExpresses GCaMP6s in genetically defined neurons expressing Cre recombinaseDepositorInsertCCaMP6s
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFPExpressionMammalianMutationPromoterhuman synaptophysinAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRX-C751-E3616S
Plasmid#182844Purposemutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG inductionDepositorInsertTRX (w Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H171
Plasmid#170353PurposemTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H170
Plasmid#170352PurposemTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b
Plasmid#109867PurposeProtein expression and purification of LRP5_Ldl-recept-bDepositorInsertLRP5_Ldl-recept-b (LRP5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b
Plasmid#109785PurposeProtein expression and purification of LRP5_Ldl-recept-bDepositorInsertLRP5_Ldl-recept-b (LRP5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:GUS
Plasmid#108444PurposeArabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertHDT2 promoter (HD2B Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterHDT2Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:HDT2-GFP
Plasmid#108566PurposeArabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in rootsDepositorInsertHDT2 (HD2B Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S828E
Plasmid#91999PurposeExpresses FLAG-HA-AGO2 (S824E-S828E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S831E
Plasmid#92000PurposeExpresses FLAG-HA-AGO2 (S824E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828E-S831E
Plasmid#92001PurposeExpresses FLAG-HA-AGO2 (S828E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS828E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S828E-S831E
Plasmid#92002PurposeExpresses FLAG-HA-AGO2 (S824E-S828E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824A-T830A
Plasmid#92003PurposeExpresses FLAG-HA-AGO2 (S824A-T830A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, T830APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOG40-HRS (257-276) Hs
Plasmid#83236PurposepGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coliDepositorInsertHRS (257-276) Hs (HGS Human)
UseTagsExpressionBacterialMutation258-277PromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 Y71A
Plasmid#71865Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Tyrosine 71 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
RalA (G23V)(mature peptide)-pcw107-V5
Plasmid#64643Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertRALA (RALA Human)
UseLentiviralTagsV5ExpressionMutationG23VPromoterPGKAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
RalA (G23V)(mature peptide)-pcw107
Plasmid#64642Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertRALA (RALA Human)
UseLentiviralTagsExpressionMutationG23VPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT
Plasmid#111398PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346APromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT
Plasmid#111399PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346DPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT
Plasmid#111400PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540APromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT
Plasmid#111451PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540DPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1
Plasmid#111462PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/S536A/L540A, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1
Plasmid#111497PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/S536D/L540D, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only