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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

Plasmid

#112987

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

S123-125-3A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

Plasmid

#112986

Purpose

P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

T331D

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MEI-S332-GFP genomic in pCaSpeR

Plasmid

#113174

Purpose

In P element vector for transposon insertion into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
6xmyc_cort_UAS-P

Plasmid

#112991

Purpose

P element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Pesin et al PLoS Genet. 2007 Nov;3(11):e202. doi: 10

Insert

cort (Cdc20) (cort Fly)

Use

P insertion vector

Tags

6X myc epitope

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Use

Tags

Expression

Bacterial

Mutation

wild-type C terminus

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C2 G10 png/GFP in casper4

Plasmid

#112970

Purpose

P element vector for transposon insertion of png-GFP into Drosophila genome

Depositor

Terry Orr-Weaver

Insert

png-GFP (png Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P-1 BICC FL

Plasmid

#112998

Purpose

For protein expression and purification of full-length Drosophila BicC

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length BicC (BicC Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu cDNA LD12084

Plasmid

#112973

Purpose

Cloning vector carrying the gnu cDNA

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

Plasmid

#112988

Purpose

P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

T331A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P-1 PUM FL

Plasmid

#112997

Purpose

For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

pumilio (pum Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

amino acids 1-355

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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C2 H5 png-Ha/His in Casper

Plasmid

#112969

Purpose

P element vector for transposon insertion of png-HA/His into Drosophila genome

Depositor

Terry Orr-Weaver

Insert

png-HA/His (png Fly)

Use

P insertion vector

Tags

HA/His epitope

Expression

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

Plasmid

#112985

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

S123-125-3D

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL c2x Tral FL-15A

Plasmid

#113011

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to A

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge